De novo transcriptome sequencing and analysis of freshwater snail (Radix balthica) to discover genes and pathways affected by exposure to oxazepam

Autor: Florence Geret, Frédéric Escudier, Elsa Bonnafé, Christophe Klopp, Jean-Yves Mazzitelli
Přispěvatelé: Biochimie et Toxicologie des Substances Bioactives (BTSB), Institut national universitaire Champollion [Albi] (INUC), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRA), Institut National de la Recherche Agronomique (INRA), Region Midi-Pyrenees, Toulouse (France), Jean-Francois Champollion University Institute, Albi (France)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Differential Analysis
Health
Toxicology and Mutagenesis
Snails
Gene Expression
Fresh Water
010501 environmental sciences
Management
Monitoring
Policy and Law
Toxicology
Ecotoxicology
01 natural sciences
Deep sequencing
Freshwater snail
Transcriptome
03 medical and health sciences
[SDV.SP.MED]Life Sciences [q-bio]/Pharmaceutical sciences/Medication
Radix balthica
medicine
Animals
0105 earth and related environmental sciences
Genetics
Psychotropic Drugs
biology
Oxazepam
Ecology
Hatching
Gene Expression Profiling
High-Throughput Nucleotide Sequencing
RNA sequencing
General Medicine
biology.organism_classification
[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]
6. Clean water
Gene expression profiling
[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology
030104 developmental biology
Psychotropic drug
Pharmaceutical
[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
medicine.drug
Zdroj: Ecotoxicology
Ecotoxicology, Springer Verlag, 2017, 26 (1), pp.127-140. ⟨10.1007/s10646-016-1748-1⟩
ISSN: 0963-9292
1573-3017
Popis: Pharmaceuticals are increasingly found in aquatic ecosystems due to the non-efficiency of waste water treatment plants. Therefore, aquatic organisms are frequently exposed to a broad diversity of pharmaceuticals. Freshwater snail Radix balthica has been chosen as model to study the effects of oxazepam (psychotropic drug) on developmental stages ranging from trochophore to hatching. In order to provide a global insight of these effects, a transcriptome deep sequencing has been performed on exposed embryos. Eighteen libraries were sequenced, six libraries for three conditions: control, exposed to the lowest oxazepam concentration with a phenotypic effect (delayed hatching) (TA) and exposed to oxazepam concentration found in freshwater (TB). A total of 39,759,772 filtered raw reads were assembled into 56,435 contigs having a mean length of 1579.68 bp and mean depth of 378.96 reads. 44.91% of the contigs have at least one annotation. The differential expression analysis between the control condition and the two exposure conditions revealed 146 contigs differentially expressed of which 144 for TA and two for TB. 34.0% were annotated with biological function. There were four mainly impacted processes: two cellular signalling systems (Notch and JNK) and two biosynthesis pathways (Polyamine and Catecholamine pathways). This work reports a large-scale analysis of differentially transcribed genes of R. balthica exposed to oxazepam during egg development until hatching. In addition, these results enriched the de novo database of potential ecotoxicological models.
Databáze: OpenAIRE
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