Leptin immunoexpression and innervation in rat interscapular brown adipose tissue of cold-acclimated rats: the effects of L-arginine and L-NAME
| Autor: | Aleksandra Korac, Aleksandra Jankovic, Biljana Buzadzic, V.M. Petrović, Bato Korac, Ana Vasilijević |
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| Rok vydání: | 2008 |
| Předmět: |
Leptin
Male Cell physiology medicine.medical_specialty Histology Arginine Acclimatization Adipose tissue Biology Pathology and Forensic Medicine Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Adipose Tissue Brown Internal medicine Brown adipose tissue medicine Animals lcsh:QH573-671 030304 developmental biology 0303 health sciences lcsh:Cytology digestive oral and skin physiology General Medicine Immunohistochemistry Rats 3. Good health Cold Temperature NG-Nitroarginine Methyl Ester Endocrinology medicine.anatomical_structure chemistry Cytoplasm 030217 neurology & neurosurgery |
| Zdroj: | Folia Histochemica et Cytobiologica Folia Histochemica et Cytobiologica, Vol 46, Iss 1, Pp 103-109 (2008) |
| ISSN: | 1897-5631 0239-8508 |
| DOI: | 10.2478/v10042-008-0015-6 |
| Popis: | The aim of the present study was to explore the effect of nitric oxide on leptin immunoexpression and innervation in interscapular brown adipose tissue (IBAT) of room-and cold-acclimated rats. Animals acclimated both to room-temperature (22 +/- 1 degrees C) and cold (4 +/- 1 degrees C) were treated with L-arginine, a substrate for nitric oxide synthases (NOSs), or N?-nitro-L-arginine methyl ester (L-NAME), an inhibitor of NOSs, for 45 days. Leptin expression and localization in brown adipocytes was studied by immunohistochemistry, and innervation stained by the Bodian method. Strong leptin immunopositivity was observed in brown adipocytes cytoplasm of all room-acclimated groups, but nuclear leptin positivity was found only in L-NAME treated rats. In cold-acclimated control and L-NAME treated rats leptin immunopositivity was absent, while L-arginine treatment reversed the cold-induced suppression of leptin expression. Comparing to control, L-arginine, and even more L-NAME, at 22 +/- 1 degrees C induced greater innervation. In conclusion, L-arginine treatment changes leptin expression pattern on cold in rat IBAT. null |
| Databáze: | OpenAIRE |
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