Livers provide a reliable matrix for real-time PCR confirmation of avian botulism
| Autor: | Hervé Morvan, Marie-Agnès Baudouard, Marie-Hélène Bayon-Auboyer, Caroline Le Maréchal, Sandra Rouxel, Marianne Chemaly, Rozenn Souillard, Typhaine Poezevara, Emmanuelle Houard, Patrick Fach, Sophie Le Bouquin, Valentine Ballan, Cédric Woudstra |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences 030106 microbiology Prevalence Biology medicine.disease_cause Real-Time Polymerase Chain Reaction Microbiology 0403 veterinary science 03 medical and health sciences Mice medicine Clostridium botulinum Animals Botulism Avian botulism Pcr analysis Bird Diseases Outbreak 04 agricultural and veterinary sciences medicine.disease Virology Infectious Diseases Real-time polymerase chain reaction Liver Cloaca |
| Zdroj: | Anaerobe. 38 |
| ISSN: | 1095-8274 |
| Popis: | Diagnosis of avian botulism is based on clinical symptoms, which are indicative but not specific. Laboratory investigations are therefore required to confirm clinical suspicions and establish a definitive diagnosis. Real-time PCR methods have recently been developed for the detection of Clostridium botulinum group III producing type C, D, C/D or D/C toxins. However, no study has been conducted to determine which types of matrices should be analyzed for laboratory confirmation using this approach. This study reports on the comparison of different matrices (pooled intestinal contents, livers, spleens and cloacal swabs) for PCR detection of C. botulinum. Between 2013 and 2015, 63 avian botulism suspicions were tested and 37 were confirmed as botulism. Analysis of livers using real-time PCR after enrichment led to the confirmation of 97% of the botulism outbreaks. Using the same method, spleens led to the confirmation of 90% of botulism outbreaks, cloacal swabs of 93% and pooled intestinal contents of 46%. Liver appears to be the most reliable type of matrix for laboratory confirmation using real-time PCR analysis. |
| Databáze: | OpenAIRE |
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