Site-Specific Surface Functionalization via Microchannel Cantilever Spotting (µCS): Comparison between Azide-Alkyne and Thiol-Alkyne Click Chemistry Reactions
| Autor: | Seyed Mohammad Mahdi Dadfar, Uwe Bog, Michael Hirtz, Sylwia Sekula-Neuner, Vanessa Trouillet |
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| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Microchannel Alkyne 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Combinatorial chemistry 0104 chemical sciences Biomaterials chemistry.chemical_compound chemistry Covalent bond Click chemistry Thiol Surface modification General Materials Science Azide 0210 nano-technology Microscale chemistry Biotechnology |
| Zdroj: | Small. 14:1800131 |
| ISSN: | 1613-6810 |
| DOI: | 10.1002/smll.201800131 |
| Popis: | Different types of click chemistry reactions are proposed and used for the functionalization of surfaces and materials, and covalent attachment of organic molecules. In the present work, two different catalyst-free click approaches, namely azide-alkyne and thiol-alkyne click chemistry are studied and compared for the immobilization of microarrays of azide or thiol inks on functionalized glass surfaces. For this purpose, the surface of glass is first functionalized with dibenzocyclooctyne-acid (DBCO-acid), a cyclooctyne with a carboxyl group. Then, the DBCO-terminated surfaces are functionalized via microchannel cantilever spotting with different fluorescent and nonfluorescent azide and thiol inks. Although both routes work reliably for surface functionalization, the protein binding experiments reveal that using a thiol-alkyne route will obtain the highest surface density of molecular immobilization in such spotting approaches. The obtained achievements and results from this work can be used for design and manufacturing of microscale patterns suitable for biomedical and biological applications. |
| Databáze: | OpenAIRE |
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