HIV type 1 pol gene diversity and antiretroviral drug resistance mutations in the Democratic Republic of Congo (DRC)
| Autor: | Diane Valéa, S. Edidi Bazepeo, Mulowayi Kashi, Jean-Willy Tshimpaka, François Lepira, Claire Mulanga, Nicole Vidal, Martine Peeters, J. Kasali Mwamba, Eric Delaporte, N. Mama |
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| Rok vydání: | 2006 |
| Předmět: |
Genetics
Recombination Genetic Pol genes biology Immunology Human immunodeficiency virus (HIV) virus diseases Antiretroviral drug biology.organism_classification medicine.disease_cause Virology Genes pol Virus Infectious Diseases parasitic diseases Lentivirus Drug Resistance Viral Mutation medicine Democratic Republic of the Congo HIV-1 Gene |
| Zdroj: | AIDS research and human retroviruses. 22(2) |
| ISSN: | 0889-2229 |
| Popis: | To study recombination and the natural polymorphism in pol of HIV-1 strains in the Democratic Republic of Congo (DRC) we sequenced the protease and RT genes for 70 HIV-1 strains previously characterized in the env V3-V5 region from a sentinel surveillance study in 2002. For 41 of the 70 (58.6%) strains, the same subtype/ CRF designations were observed in pol and env. Twenty-three (32.9%) of 70 pol sequences were complex recombinants involving two to five subtypes as well as fragments that could not be classified into any of the known subtypes. All subtypes were involved in recombination events. Unclassified (U) and env subtype H strains were very likely to be recombinant strains. Overall, many minor mutations were identified in the protease sequences. Although at the time of our study ARV use was not yet widespread in DRC, three strains were identified with one major mutation associated with drug resistance: L90M and M46L in protease and K103N in RT. |
| Databáze: | OpenAIRE |
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