In vitro antioxidant activity of polyphenol extracts with antiviral properties from Geranium sanguineum L

Autor: Ekaterina Krumova, Atalay Sokmen, Julia Serkedjieva, Stefka Ivancheva, Maria Angelova, Münevver Sökmen, Svetlana Pashova
Přispěvatelé: Bulgarian Acad Sci, Inst Microbiol, BU-1113 Sofia, Bulgaria -- Cumhuriyet Univ, Fac Sci & Literature, Dept Biol, Sivas, Turkey -- Bulgarian Acad Sci, Inst Bot, BU-1113 Sofia, Bulgaria -- Cumhuriyet Univ, Fac Sci & Literature, Dept Chem, Sivas, Turkey
Rok vydání: 2005
Předmět:
Antioxidant
Geranium
medicine.medical_treatment
antioxidant effect
medicine.disease_cause
Antioxidants
Mice
chemistry.chemical_compound
Cytopathogenic Effect
Viral
Superoxides
Caffeic acid
Influenza A virus
General Pharmacology
Toxicology and Pharmaceutics
Bulgaria
Cells
Cultured
Traditional medicine
biology
Free Radical Scavengers
General Medicine
beta Carotene
Biphenyl compound
Hemagglutinins
Hydrazines
Biochemistry
Spectrophotometry
polyphenol extracts
Geranium sanguineum
Linoleic acid
Enzyme-Linked Immunosorbent Assay
radical scavenging activity
Antiviral Agents
General Biochemistry
Genetics and Molecular Biology
Linoleic Acid
Dogs
Orthomyxoviridae Infections
Phenols
Picrates
In vivo
medicine
Animals
Flavonoids
Dose-Response Relationship
Drug
Plant Extracts
Hemagglutination
influenza virus inhibition
Biphenyl Compounds
Polyphenols
biology.organism_classification
chemistry
Polyphenol
Zdroj: Life Sciences. 76:2981-2993
ISSN: 0024-3205
Popis: WOS: 000228593700009
PubMed ID: 15820508
Recent evidence shows that plant polyphenols exhibit antioxidant and radical scavenging properties. By three separate and complementary methods - DPPH assay, beta-carotene-linoleic acid assay and NBT-reduction assay it was established that a polyphenol-rich extract from the medicinal plant Geranium sanguineum L. with strong anti-influenza virus activity, possessed antioxidant and radical scavenging capacities. For comparative reasons caffeic acid and the synthetic antioxidant BHT were used. Total soluble phenolic constituents of the MeOH extract measured by Folin-Ciocalteu reagent were found as 34.60% (w/w). Further it was demonstrated that the EtOAc fraction, retaining the majority of the in vivo protective effect exhibited a strong O-2(-) scavenging activity while the n-BuOH fraction, containing the majority of the in vitro antiviral activity provoked generation of O-2(-). The O-2(-) scavenging activity of all three preparations correlated with the rate of the protective effect shown in the murine model of experimental influenza virus infection. The present results are in accordance with our intensive studies on the mode of the protective effect of the plant extract which showed positively that the protection may possibly be attributed to the combination of more than one biological activities and that the use of antioxidants might be an useful approach in the treatment of influenza infection. (c) 2005 Elsevier Inc. All rights reserved.
Databáze: OpenAIRE
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