Dietary hydrolyzed wheat gluten supplementation ameliorated intestinal barrier dysfunctions of broilers challenged with Escherichia coli O78
| Autor: | In Ho Kim, Yujun Wu, Ying Yang, Wenyu Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
HWG
hydrolyzed wheat gluten IL-1β interleukin-1β Glutens inflammatory cytokines tight junction NRC National Research Council Wheat gluten Spleen Occludin medicine.disease_cause SF1-1100 digestive system TNF-α tumor necrosis factor-α ZO-1 zonula occluden-1 TLR4 Toll like receptor-4 Hydrolysis DAO diamine oxidase IMMUNOLOGY HEALTH AND DISEASE medicine Escherichia coli Animals IFN-γ interferon-γ Food science Intestinal Mucosa IL-6 interleukin-6 Escherichia coli Infections Triticum IL-8 interleukin-8 Glu glutamate Tight junction Chemistry intestinal permeability Gly glycine General Medicine Animal Feed Animal culture Diet medicine.anatomical_structure Mrna level GAPDH glyceraldehyde-3-phosphate dehydrogenase Dietary Supplements Gln glutamine Animal Science and Zoology Diamine oxidase Chickens hydrolyzed wheat gluten |
| Zdroj: | Poultry Science Poultry Science, Vol 101, Iss 2, Pp 101615-(2022) |
| ISSN: | 1525-3171 |
| Popis: | This study aimed to investigate a protective effect of hydrolyzed wheat gluten (HWG) on Escherichia coli (E. coli)-induced intestinal barrier dysfunctions in broilers. Broilers fed a basal diet unsupplemented or supplemented with HWG (0.5% or 1%) were intraperitoneally injected with either E. coli O78 suspension (108 CFU/mL) or equal volume of vehicle on d 18 of age. Blood and tissue samples were collected 3rd d post infection. The results showed that E. coli-infection increased immune-organ indexes of spleen and thymus, enhanced serum diamine oxidase (DAO) level, impaired ileal villus structure and reduced tight junction mRNA levels (Occludin, Claudin-1, ZO-1, P < 0.05), while increased mRNA levels of inflammatory cytokines (TNF-α, IFN-γ, IL-1β, IL-6, IL-8) and TLR4 in the ileum of broilers (P < 0.05). The effects of E. coli O78 challenge on organ indexes of spleen and thymus, serum DAO level, mRNA levels of tight junctions were alleviated by 1% HWG supplementation, the upregulations of IL-1β and TLR4 were prevented by 0.5% HWG supplementation (P < 0.05). In addition, increased IFN-γ of E. coli-infected broilers was abrogated by 0.5% or 1% HWG supplementation (P < 0.05). In summary, dietary HWG supplementation ameliorated intestinal barrier dysfunctions triggered by E. coli-infection in the ileum of broilers. HWG supplementation might be a nutritional strategy to improve the intestinal mucosal barrier function of broilers. |
| Databáze: | OpenAIRE |
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