Comparison of species identification of endocarditis associated viridans streptococci using rnpB genotyping and 2 MALDI-TOF systems
| Autor: | Bo Nilson, Björn Herrmann, Magnus Rasmussen, Lars Olaison, Elisabeth Ek, Liselott Svensson Stadler, Siri Kurland, Jenny Isaksson |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
biology Genotyping Techniques Streptococcus Streptococcus gordonii General Medicine Endocarditis Bacterial Streptococcus cristatus medicine.disease_cause biology.organism_classification Viridans Streptococci Sensitivity and Specificity Microbiology Streptococcus sanguinis Infectious Diseases Streptococcus oralis Viridans streptococci Streptococcus mitis Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Streptococcal Infections Streptococcus tigurinus medicine Humans |
| Zdroj: | Diagnostic microbiology and infectious disease. 81(4) |
| ISSN: | 1879-0070 |
| Popis: | Streptococcus spp. are important causes of infective endocarditis but challenging in species identification. This study compared identification based on sequence determination of the rnpB gene with 2 systems of matrix-assisted laser desorption ionization-time of flight mass spectrometry, MALDI Biotyper (Bruker) and VITEK MS IVD (bioMerieux). Blood culture isolates of viridans streptococci from 63 patients with infective endocarditis were tested. The 3 methods showed full agreement for all 36 isolates identified in the Anginosus, Bovis, and Mutans groups or identified as Streptococcus cristatus, Streptococcus gordonii, or Streptococcus sanguinis. None of the methods could reliably identify the 23 isolates to the species level when designated as Streptococcus mitis, Streptococcus oralis, or Streptococcus tigurinus. In 7 isolates classified to the Mitis group, the rnpB sequences deviated strikingly from all reference sequences, and additional analysis of sodA and groEL genes indicated the occurrence of yet unidentified Streptococcus spp. |
| Databáze: | OpenAIRE |
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