Genetic diversity and hematological and biochemical alterations in Alouatta primates naturally infected with hemoplasmas in Brazil
| Autor: | Cristiane Maria Fernandes de Melo, Marcos Rogério André, Edmilson Rodrigo Daneze, Simone de Jesus Fernandes, Márcia Ferreira da Rosa Sobreira, Natalia Serra Mendes, Jorge Alfonso Morales-Donoso, Inalda Angélica de Souza Ramos, Rosangela Zacarias Machado |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Ctr Univ Moura Lacerda |
| Rok vydání: | 2018 |
| Předmět: |
Bartonella
DNA Bacterial Immunology Zoology Captivity Howler monkeys Biology medicine.disease_cause Microbiology Genetic diversity Mycoplasma Bartonella Infections RNA Ribosomal 16S parasitic diseases medicine Immunology and Allergy Animals Mycoplasma Infections Gene Alouatta General Veterinary Bartonellosis Monkey Diseases Genetic Variation General Medicine South America medicine.disease 16S ribosomal RNA biology.organism_classification Infectious Diseases Hemotrophic mycoplasmas Mammal Brazil |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1878-1667 |
| Popis: | Made available in DSpace on 2019-10-04T12:37:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-04-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Mycoplasma spp. and Bartonella spp. are Gram-negative bacteria transmitted by arthropod vectors that infect red blood cells of several mammal species. This study investigated the occurrence and genetic diversity of hemoplasmas and Bartonella spp. in 68 howler monkeys kept in captivity in Sao Paulo, a southeastern state in Brazil. In addition, possible hematological, biochemical and electrophoretic changes of serum proteins associated with the occurrence of hemoplasmas and Bartonella spp. in captive primates were also investigated. The cPCR results showed that all sampled howler monkeys were negative for Bartonella spp. based on the gltA gene. The cPCR results indicated that 18 (26.47%) non-human primates (NHP) were positive for hemoplasmas based on the 16S rRNA gene. Monocyte and lymphocyte counts were higher in hemoplasma-positive howlers (P < 0.05). Platelet counts decreased in nonhuman primates (NHP) positive for hemoplasmas (P < 0.05). The results from the blood serum proteinogram and biochemistry analyses were not significantly different between NHPs positive and negative for hemotrophic mycoplasmas. Phylogenetic analysis using Bayesian Inference (BI) based on the 16S rRNA gene positioned the obtained sequences close to 'Candidatus Mycoplasma kahanei'. The analysis of sequence diversity of the 16S rRNA gene showed that 5 different genotypes are circulating in NHP in Brazil and in the world; besides, a clear separation between the sequences of hemoplasmas that infect NHP of the Sapajus and Alouatta genus in Brazil was found, probably corresponding to two different species. The pathogenic potential of this hemoplasma species in NHP should be further investigated. Univ Estadual Paulista, Dept Vet Pathol, Lab Immunoparasitol, Jaboticabal, SP, Brazil Univ Estadual Paulista, Dept Genet & Anim Breeding, Jaboticabal, SP, Brazil Ctr Univ Moura Lacerda, Dept Vet Clin Pathol, Ribeirao Preto, SP, Brazil Univ Estadual Paulista, Dept Vet Pathol, Lab Immunoparasitol, Jaboticabal, SP, Brazil Univ Estadual Paulista, Dept Genet & Anim Breeding, Jaboticabal, SP, Brazil CNPq: 302420/2017-7 |
| Databáze: | OpenAIRE |
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