Folate-conjugated iron oxide nanoparticles for solid tumor targeting as potential specific magnetic hyperthermia mediators: synthesis, physicochemical characterization, and in vitro experiments
| Autor: | Paolo Colombo, Sébastien Vasseur, Stéphane Mornet, Fabio Sonvico, Etienne Duguet, Johan Hoebeke, Danielle Jaillard, Patrick Couvreur, Catherine Dubernet, Jeril Degrouard |
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| Přispěvatelé: | Physico-chimie, pharmacotechnie, biopharmacie (PCPB), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Dipartimento Farmaceutico, University of Parma = Università degli studi di Parma [Parme, Italie], Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Université de Bordeaux (UB), Développement et évolution (DE), Immunologie et chimie thérapeutiques (ICT), Cancéropôle du Grand Est-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2005 |
| Předmět: |
Hot Temperature
Pharmaceutical Science Nanoparticle 02 engineering and technology Sodium Chloride 01 natural sciences Ferric Compounds chemistry.chemical_compound Neoplasms Intracellular hyperthermia Diagnosis Surface plasmon resonance Liposome Chemistry [CHIM.MATE]Chemical Sciences/Material chemistry Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Endocytosis Biochemistry Folate receptor Organ Specificity 0210 nano-technology Iron oxide nanoparticles Biotechnology Superparamagnetism Receptor Fever Cells Static Electricity Biomedical Engineering Bioengineering 010402 general chemistry Magnetics Folic Acid Microscopy Electron Transmission Cell Line Tumor Humans Particle Size Dextran Pharmacology Delivery drugs Organic Chemistry Osmolar Concentration Surface Plasmon Resonance 0104 chemical sciences Nanostructures Magnetic hyperthermia Liposomes Surface modification Carriers Therapy |
| Zdroj: | Bioconjugate Chemistry Bioconjugate Chemistry, American Chemical Society, 2005, vol. 16, n° 5, p. 1181-1188. ⟨10.1021/bc050050z⟩ |
| ISSN: | 1043-1802 1520-4812 |
| DOI: | 10.1021/bc050050z⟩ |
| Popis: | New folate-conjugated superparamagnetic maghemite nanoparticles have been synthesized for the intracellular hyperthermia treatment of solid tumors. These ultradispersed nanosystems have been characterized for their physicochemical properties and tumor cell targeting ability, facilitated by surface modification with folic acid. Preliminary experiments of nanoparticles heating under the influence of an alternating magnetic field at 108 kHz have been also performed. The nanoparticle size, surface charge, and colloidal stability have been assessed in various conditions of ionic strength and pH. The ability of these folate "decorated" maghemite nanoparticles to recognize the folate receptor has been investigated both by surface plasmon resonance and in folate receptor expressing cell lines, using radiolabeled folic acid in competitive binding experiments. The specificity of nanoparticle cellular uptake has been further investigated by transmission electron microscopy after incubation of these nanoparticles in the presence of three cell lines with differing folate receptor expression levels. Qualitative and quantitative determinations of both folate nanoparticles and nontargeted control nanoparticles demonstrated a specific cell internalization of the folate superparamagnetic nanoparticles. |
| Databáze: | OpenAIRE |
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