A proteomics sample preparation method for mature, recalcitrant leaves of perennial plants
| Autor: | Liu Lijun, Wang Bo, Deng Gang, Zhang Na, Zhong Xinyue, Peng Ding-xiang, Lao Chengying |
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| Rok vydání: | 2014 |
| Předmět: |
Proteomics
lcsh:Medicine Analytic Sample Preparation Methods Crops Orange (colour) Biology Biochemistry Ramie Protein purification Lysis buffer Botany Sample preparation lcsh:Science Plant Proteins Gel electrophoresis PEAR Multidisciplinary Chromatography lcsh:R Biology and Life Sciences Agriculture Plants Plant Leaves lcsh:Q Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 7, p e102175 (2014) |
| ISSN: | 1932-6203 |
| Popis: | Sample preparation is key to the success of proteomics studies. In the present study, two sample preparation methods were tested for their suitability on the mature, recalcitrant leaves of six representative perennial plants (grape, plum, pear, peach, orange, and ramie). An improved sample preparation method was obtained: Tris and Triton X-100 were added together instead of CHAPS to the lysis buffer, and a 20% TCA-water solution and 100% precooled acetone were added after the protein extraction for the further purification of protein. This method effectively eliminates nonprotein impurities and obtains a clear two-dimensional gel electrophoresis array. The method facilitates the separation of high-molecular-weight proteins and increases the resolution of low-abundance proteins. This method provides a widely applicable and economically feasible technology for the proteomic study of the mature, recalcitrant leaves of perennial plants. |
| Databáze: | OpenAIRE |
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