Synthesis and characterization of gold nanostructured Chorin e6 for Photodynamic Therapy
| Autor: | K. C. Hewitt, Maiara L. Castilho, Juliana Ferreira-Strixino, Leandro Raniero, Laís de Souza Vieira, Isabelle Ferreira |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030103 biophysics Porphyrins Cell Survival medicine.medical_treatment Biophysics Metal Nanoparticles Photodynamic therapy 02 engineering and technology Dermatology Photochemistry 03 medical and health sciences chemistry.chemical_compound Cell Line Tumor Zeta potential medicine Humans Pharmacology (medical) Photosensitizer Fourier transform infrared spectroscopy Cytotoxicity Photosensitizing Agents Chlorophyllides Dose-Response Relationship Drug Chemistry Dose-Response Relationship Radiation Neoplasms Experimental 021001 nanoscience & nanotechnology Treatment Outcome Photochemotherapy Oncology Thiourea Colloidal gold Trypan blue Gold 0210 nano-technology |
| Zdroj: | Photodiagnosis and Photodynamic Therapy. 18:6-11 |
| ISSN: | 1572-1000 |
| DOI: | 10.1016/j.pdpdt.2016.12.012 |
| Popis: | Photodynamic therapy is an alternative treatment for cancer based on cellular uptake of a photosensitizer, illuminated with an appropriate wavelength in the presence of oxygen. A cascade of reactions generates reactive oxygen species leading to cell death. Using carbodiimide chemistry, chlorin e6 (Ce6) was covalently bonded to thiourea, and (via the sulphur end group) to gold nanoparticles (AuNPs), forming the Ce6-AuNP complex. Ce6 absorbs in the range 650–680 nm, where the coefficient of biological tissue absorption is low (part of the therapeutic window), which is ideal for biological application. Transmission Electron Microscopy, UV–vis spectroscopy, Fourier transform Infrared Spectroscopy and Zeta potential measurements were completed to characterize the Ce6-AuNP complex. The bare AuNPs have an average diameter of 18 ± 4 nm. A line of human breast carcinoma cells (MDA-MB-468) was used to determine whether Ce6 functionalization to AuNPs potentiate its activity. Trypan blue assays were used to assess cell viability. In the absence of light, Ce6 either alone or bounded to AuNPs was not cytotoxic. When irradiated at 660 nm, the cytotoxicity of Ce6-AuNP was higher than Ce6 alone for MDA-MB-468 cells using 4 h incubation. AuNPs without Ce6 showed no cytotoxic. |
| Databáze: | OpenAIRE |
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