Prefabrication and free transfer of a tissue engineered composite flap - An experimental model in the rat
| Autor: | Lukas Prantl, Mihai Ionac, Rainer Staudenmaier, Lucian P. Jiga, Bogdan Hoinoiu, Veronika Mandlik, Andreas Kehrer, Friedrich Jung |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Scaffold Materials science Physiology medicine.medical_treatment Skin flap 030230 surgery Surgical Flaps Prefabrication 03 medical and health sciences 0302 clinical medicine Tissue engineering Physiology (medical) medicine Animals Rats Wistar Tissue engineered Groin Tissue Engineering Experimental model Hematology Skin Transplantation Microsurgery Rats Disease Models Animal medicine.anatomical_structure 030220 oncology & carcinogenesis Cardiology and Cardiovascular Medicine Biomedical engineering |
| Zdroj: | Clinical hemorheology and microcirculation. 64(3) |
| ISSN: | 1875-8622 |
| Popis: | Background The technique of flap-prefabrication has been successfully established in tissue engineering: missing intrinsic vascularisation of engineered tissue can be generated in vivo by microsurgical vesselloop construction. It is possible to move engineered tissue into a defect with microsurgery. In the literature, the combination of engineered tissue covered with skin is not widely reported. Objective Aim of this study was to establish a model to investigate scaffold prefabrication with full thickness skin graft coverage with subsequent free tissue transfer. Methods 8 Wistar rats were operated in 2 separate steps: 1) after creating an arteriovenous loop with the femoral vessels, a porous scaffold was placed on the loop and covered with an inguinally based skin flap. A control was implanted without loop into the contralateral groin. 2) 6 weeks later the prefabricated composite flaps were microsurgically transferred to the cervical region. Skin-island monitoring was performed with Laser Doppler-scanner after the transfer. Results Continuous loss of the skin islands was observed within 72 hours. Complications included wound-dehiscence, thrombosis and death from anaesthesia; in spite of consistent loop viability. Conclusion Evaluation showed that modifications are necessary to maintain the skin-island cove. |
| Databáze: | OpenAIRE |
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