Functional proteomic analysis of human nucleolus
| Autor: | Karine Kindbeiter, Denis F. Hochstrasser, Yohann Couté, Aleth Callé, Jean-Charles Sanchez, Catherine Déon, Jean-Jacques Diaz, Anna Greco, Alexander Scherl |
|---|---|
| Přispěvatelé: | Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
MESH: Databases
Protein Proteome Nucleolus Ribosome biogenesis [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Nuclear Proteins/analysis/classification Peptide Mapping MESH: Peptide Mapping Article 03 medical and health sciences Transcription (biology) Gene expression Humans Nuclear protein ddc:576 Databases Protein Molecular Biology 030304 developmental biology 0303 health sciences MESH: Humans Peptide mapping 030302 biochemistry & molecular biology Nuclear Proteins Computational Biology Cell Biology Cell biology MESH: Hela Cells MESH: Proteome Nucleolar Proteins Cell Nucleolus/chemistry/ultrastructure MESH: Cell Nucleolus MESH: Nuclear Proteins Cell Nucleolus HeLa Cells MESH: Computational Biology |
| Zdroj: | Molecular Biology of the Cell Molecular Biology of the Cell, American Society for Cell Biology, 2002, 13 (11), pp.4100-9. ⟨10.1091/mbc.E02-05-0271⟩ Molecular Biology of the Cell, Vol. 13, No 11 (2002) pp. 4100-9 |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.E02-05-0271⟩ |
| Popis: | The notion of a “plurifunctional” nucleolus is now well established. However, molecular mechanisms underlying the biological processes occurring within this nuclear domain remain only partially understood. As a first step in elucidating these mechanisms we have carried out a proteomic analysis to draw up a list of proteins present within nucleoli of HeLa cells. This analysis allowed the identification of 213 different nucleolar proteins. This catalog complements that of the 271 proteins obtained recently by others, giving a total of ∼350 different nucleolar proteins. Functional classification of these proteins allowed outlining several biological processes taking place within nucleoli. Bioinformatic analyses permitted the assignment of hypothetical functions for 43 proteins for which no functional information is available. Notably, a role in ribosome biogenesis was proposed for 31 proteins. More generally, this functional classification reinforces the plurifunctional nature of nucleoli and provides convincing evidence that nucleoli may play a central role in the control of gene expression. Finally, this analysis supports the recent demonstration of a coupling of transcription and translation in higher eukaryotes. |
| Databáze: | OpenAIRE |
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