Separation of proteins by reversed-phase high-performance liquid chromatography
| Autor: | W.G. Burton, L.R. Snyder, B.F. Johnson, K.D. Nugent, Timothy Slattery |
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| Rok vydání: | 1988 |
| Předmět: |
Chromatography
Monolithic HPLC column Hydrophilic interaction chromatography Organic Chemistry Analytical chemistry Fast protein liquid chromatography Reversed-phase chromatography General Medicine Biochemistry High-performance liquid chromatography Analytical Chemistry Chiral column chromatography Chaotropic agent chemistry.chemical_compound Countercurrent chromatography chemistry Two-dimensional chromatography Chromatography detector Phase (matter) Sodium dodecyl sulfate Chromatography column |
| Zdroj: | Journal of Chromatography A. 443:363-379 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(00)94808-8 |
| Popis: | In the process of developing a new analytical technology (the chromatographoresis process®) which couples reversed-phase high-performance liquid chromatography (HPLC) to sodium dodecyl sulfate polyacrylamide gel electrophoresis in a real-time automated system, it was apparent that improvements in resolving power for the first-dimension (HPLC) separation were necessary. The present paper describes the optimization of the column for our initial work on reversed-phase HPLC separations. Polymeric (polystyrene) packings having particle diameters of 5 μm and pore diameters of 300 A were generally superior in terms of resolution, sample recovery and minimization of “ghosting”. Optimum column dimensions were 50 × 1.0 mm I.D. for the flow-rates required in our system (10–100 μl/min). |
| Databáze: | OpenAIRE |
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