Effect of cold preservation on intracellular calcium concentration and calpain activity in rat sinusoidal endothelial cells
| Autor: | Steven M. Strasberg, G. Aravinda Upadhya, John Anagli, Stefan A. Topp, Richard S. Hotchkiss |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Proteasome Endopeptidase Complex Cell chemistry.chemical_element Calcium Biology Calcium in biology Dantrolene Rats Sprague-Dawley Multienzyme Complexes medicine Animals Egtazic Acid Actin Cells Cultured Chelating Agents Cryopreservation Hepatology Calpain Osmolar Concentration Biological activity Intracellular Membranes Calcium Channel Blockers Cathepsins Cell biology Rats Endothelial stem cell Cysteine Endopeptidases medicine.anatomical_structure chemistry Biochemistry biology.protein Endothelium Vascular medicine.drug Liver Circulation |
| Zdroj: | Hepatology (Baltimore, Md.). 37(2) |
| ISSN: | 0270-9139 |
| Popis: | This study was performed to determine the role of intracellular calcium concentration and calpain activity on the cellular events that occur in rat sinusoidal endothelial cells (SEC) in the cold. Intracellular calcium concentrations were measured in isolated cold preserved rat SEC. Dantrolene or 1,2-bis(o-Aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetra(acetoxymethyl) ester (BAPTA-AM) was added in some studies. In other studies, calpain activity and m-calpain and μ-calpain expression were measured during cold preservation in the presence or absence of calpain inhibitors. The effect of addition of dantrolene to preservation solutions on function of whole livers after preservation was determined. Cold preservation caused an increase in intracellular calcium concentration first detected at 1 hour of preservation. This was associated with cell rounding and actin disassembly. Dantrolene and BAPTA-AM prevented the increase in intracellular calcium concentration and reduced cell rounding and actin disassembly. Cold preservation also resulted in increased calpain activity and expression on SEC. Calpain expression was reduced by dantrolene. Calpain inhibitors N-acetyl-leu-leu-norleucinal (ALLN) and N-acetyl-leu-leu-methioninal (ALLM) reduced calpain activity and expression and restored SEC cell shape and actin morphology. Dantrolene improved function of livers preserved in Eurocollins (EC) solution when tested on the isolated perfused rat liver (IPRL). In conclusion, exposure of SEC to cold results sequentially in elevated intracellular calcium concentration, increased calpain activity, and actin disassembly. (H EPATOLOGY 2003;37:313-323.) |
| Databáze: | OpenAIRE |
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