Protective activity of ischemic preconditioning on rat testicular ischemia: Effects of Y-27632 and 5-hydroxydecanoic acid
| Autor: | Nurten Aksoy, Abdullah Aydin, Harun Ciralik, Ahmet Gozen, Seniz Demiryürek, Abdullah Taskin, Sevgül Kara, Hasan Bilinc, Haluk Ceylan |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Pyridines Ischemia medicine.disease_cause chemistry.chemical_compound Internal medicine Testis parasitic diseases Animals Medicine cardiovascular diseases Enzyme Inhibitors Rats Wistar Ischemic Preconditioning Rho-associated protein kinase biology business.industry Antagonist General Medicine medicine.disease Amides Rats Y-27632 Endocrinology chemistry Rho kinase inhibitor Myeloperoxidase Anesthesia Pediatrics Perinatology and Child Health biology.protein Ischemic preconditioning Surgery Hydroxy Acids business Decanoic Acids Oxidative stress |
| Zdroj: | Journal of Pediatric Surgery. 48:1565-1572 |
| ISSN: | 0022-3468 |
| DOI: | 10.1016/j.jpedsurg.2012.10.074 |
| Popis: | Background/Purpose The aim of this study was to investigate the role of ischemic preconditioning (IPC) on ischemia/reperfusion (I/R)-induced injury of rat testis and determine the effects of 5-hydroxydecanoic acid (5-HD), a selective K ATP channel antagonist, and Y-27632, a selective Rho kinase inhibitor, on IPC. Methods I/R injury was induced by 180min ischemia and 60min reperfusion of testis. There were 5 groups. Group 1 served as untreated controls. The rats in Group 2 were subjected to I/R only. In Group 3, 3cycles of IPC (5min transient ischemia plus 5min reperfusion) were performed prior to I/R. In groups 4 and 5, the rats were treated as in Group 3 but received intraperitoneal injections of 0.3mg/kg Y-27632 or 10mg/kg 5-HD prior to IPC, respectively. Results I/R led to severe histopathological lesions in the rat testis and significantly lowered the scoring. I/R resulted in significant elevation in tissue lipid peroxide levels, myeloperoxidase (MPO) activity, and total antioxidative capacity (TAC), total oxidative status, and oxidative stress index levels. Protective effects of IPC on I/R-induced testicular injury of rats were observed with the significant recovery in these biochemical parameters. Y-27632 treatment led to a significant decrease in MPO activity, but there were no significant changes in the remaining parameters. Effects of IPC were blocked by 5-HD except in the TAC levels. Conclusion Our results showed that IPC protected rat testis against I/R-induced injury via activation of K ATP channels. Additionally, Rho kinase inhibition preserved the effects of IPC in testis. |
| Databáze: | OpenAIRE |
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