Chitosan/Poly(vinylpyrrolidone) Matrices Obtained by Gamma-Irradiation for Skin Scaffolds: Characterization and Preliminary Cell Response Studies
| Autor: | João Paulo Leal, M.H. Casimiro, Luís M. A. Ferreira, S.R. Gomes, Gabriela Rodrigues |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Scaffold
Materials science Biocompatibility 02 engineering and technology macromolecular substances 010402 general chemistry lcsh:Technology 01 natural sciences Article Chitosan Contact angle chemistry.chemical_compound skin regeneration Copolymer General Materials Science lcsh:Microscopy Porosity lcsh:QC120-168.85 lcsh:QH201-278.5 lcsh:T Regeneration (biology) technology industry and agriculture porous scaffolds poly(vinylpyrrolidone) (PVP) γ-radiation 021001 nanoscience & nanotechnology 0104 chemical sciences 3. Good health chemistry Chemical engineering lcsh:TA1-2040 lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering chitosan lcsh:Engineering (General). Civil engineering (General) 0210 nano-technology Wound healing lcsh:TK1-9971 |
| Zdroj: | Materials Volume 11 Issue 12 Materials, Vol 11, Iss 12, p 2535 (2018) |
| ISSN: | 1996-1944 |
| Popis: | Several studies have shown that chitosan possesses characteristics favorable for promoting dermal regeneration and accelerated wound healing. In this work we have reported the work that has been done on the development and characterization of biocompatible and biodegradable chitosan based matrices to be used as skin scaffolds. Poly(vinylpyrrrolidone) (PVP) was used as copolymer and a two steps methodology of freeze-drying and gamma irradiation was used to obtain the porous matrices. The influence of PVP content, synthesis procedure and absorbed radiation dose on matrices&rsquo physical, chemical and structural properties was evaluated by ATR-FTIR, TGA, SEM, contact angle measurements and degradation behavior. The in vitro cellular viability and proliferation of HFFF2 fibroblast cell line was analyzed as a measure of matrices&rsquo biocompatibility and ability to assist skin regeneration. Results show that over the studied range values, gamma-radiation dose, copolymer concentration and synthesis procedure can be used to tailor the matrices&rsquo morphology in terms of porosity and surface roughness. Early results from biological assays evidence the biocompatibility of the prepared chitosan/PVP matrices since cells adhered to the surface of all matrices (chitosan/PVP (5%) &gamma irradiated at 10 kGy presents the higher cellular viability). These features show that the resultant matrices could be a potential suitable scaffold for skin tissue regeneration. |
| Databáze: | OpenAIRE |
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