A conscious mouse model of gastric ileus using clinically relevant endpoints
| Autor: | Sean J. Mulvihill, Justin D Gull, Matthew A. Firpo, Yuanlin Shao, Robert E. Glasgow, Aniko Szabo, Jeffrey D. Jackson, Michael D. Rollins |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Time Factors Ileus Consciousness medicine.medical_treatment Intraperitoneal injection Blood Pressure Motor Activity Gastroenterology Eating Mice Internal medicine medicine Animals lcsh:RC799-869 Defecation Gastrointestinal Transit Mice Inbred C3H Gastric emptying Dose-Response Relationship Drug business.industry Gastric Outlet Obstruction Stomach digestive oral and skin physiology Gastric outlet obstruction General Medicine Hepatology medicine.disease digestive system diseases Endotoxins Disease Models Animal medicine.anatomical_structure Gastric Emptying Acute pancreatitis lcsh:Diseases of the digestive system. Gastroenterology business Research Article |
| Zdroj: | BMC Gastroenterology BMC Gastroenterology, Vol 5, Iss 1, p 18 (2005) |
| ISSN: | 1471-230X |
| DOI: | 10.1186/1471-230x-5-18 |
| Popis: | Background Gastric ileus is an unsolved clinical problem and current treatment is limited to supportive measures. Models of ileus using anesthetized animals, muscle strips or isolated smooth muscle cells do not adequately reproduce the clinical situation. Thus, previous studies using these techniques have not led to a clear understanding of the pathophysiology of ileus. The feasibility of using food intake and fecal output as simple, clinically relevant endpoints for monitoring ileus in a conscious mouse model was evaluated by assessing the severity and time course of various insults known to cause ileus. Methods Delayed food intake and fecal output associated with ileus was monitored after intraperitoneal injection of endotoxin, laparotomy with bowel manipulation, thermal injury or cerulein induced acute pancreatitis. The correlation of decreased food intake after endotoxin injection with gastric ileus was validated by measuring gastric emptying. The effect of endotoxin on general activity level and feeding behavior was also determined. Small bowel transit was measured using a phenol red marker. Results Each insult resulted in a transient and comparable decrease in food intake and fecal output consistent with the clinical picture of ileus. The endpoints were highly sensitive to small changes in low doses of endotoxin, the extent of bowel manipulation, and cerulein dose. The delay in food intake directly correlated with delayed gastric emptying. Changes in general activity and feeding behavior were insufficient to explain decreased food intake. Intestinal transit remained unchanged at the times measured. Conclusion Food intake and fecal output are sensitive markers of gastric dysfunction in four experimental models of ileus. In the mouse, delayed gastric emptying appears to be the major cause of the anorexic effect associated with ileus. Gastric dysfunction is more important than small bowel dysfunction in this model. Recovery of stomach function appears to be simultaneous to colonic recovery. |
| Databáze: | OpenAIRE |
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