Isogenic-induced endothelial cells enhance osteogenic differentiation of mesenchymal stem cells on silk fibroin scaffold
| Autor: | Sindhuja D. Eswaramoorthy, Subha Narayan Rath, Gayathri Korra, Nandini Dhiman, Carlo M. Oranges, Dirk J. Schaefer, Srinivas Madduri |
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| Rok vydání: | 2019 |
| Předmět: |
Embryology
Scaffold Biomedical Engineering chemistry.chemical_element Fibroin Calcium digestive system Bone tissue engineering 03 medical and health sciences 0302 clinical medicine Osteogenesis parasitic diseases Gene expression Humans 030304 developmental biology 0303 health sciences Tissue Scaffolds Chemistry Mesenchymal stem cell Endothelial Cells Cell Differentiation Mesenchymal Stem Cells Coculture Techniques digestive system diseases Cell biology Gene Expression Regulation 030220 oncology & carcinogenesis Nanofiber Fibroins tissues |
| Zdroj: | Regenerative Medicine. 14:647-661 |
| ISSN: | 1746-076X 1746-0751 |
| DOI: | 10.2217/rme-2018-0166 |
| Popis: | Aim: We investigated the role of induced endothelial cells (iECs) in mesenchymal stem cells (MSCs)/iECs co-culture and assessed their osteogenic ability on silk fibroin nanofiber scaffolds. Methods: The osteogenic differentiation was assessed by the ALP assay, calcium assay and gene expression studies. Results: The osteogenic differentiation of the iECs co-cultures was found to be higher than the MSCs group and proximal to endothelial cells (ECs) co-cultures. Furthermore, the usage of isogenic iECs for co-culture increased the osteogenic and endothelial gene expression. Conclusion: These findings suggest that iECs mimic endothelial cells when co-cultured with MSCs and that one MSCs source can be used to give rise to both MSCs and iECs. The isogenic MSCs/iECs co-culture provides a new option for bone tissue engineering applications. |
| Databáze: | OpenAIRE |
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