The Possible Role of Nitric Oxide Pathway in Pentylenetetrazole Preconditioning Against Seizure in Mice
| Autor: | Soheil Kazemi Roodsari, Hedieh Keshavarz-Bahaghighat, Erfan Bahramnejad, Hedyeh Faghir-Ghanesefat, Nazanin Rajai, Ahmad Reza Dehpour, Tahmineh Mokhtari |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male NOS inhibitor Indazoles Stimulation Nitric Oxide Synthase Type I Pharmacology Nitric Oxide Guanidines Nitric oxide GABA Antagonists 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound Mice 0302 clinical medicine Animal model Seizures Animals Enzyme Inhibitors Seizure threshold Low dose Brain General Medicine 030104 developmental biology NG-Nitroarginine Methyl Ester chemistry Chemical agents Nitric Oxide Pathway Pentylenetetrazole 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Journal of molecular neuroscience : MN. 67(3) |
| ISSN: | 1559-1166 |
| Popis: | Preconditioning is defined as an induction of adaptive response in organs against lethal stimulation provoked by subsequent mild sublethal stress. Several chemical agents have been demonstrated to cause brain tolerance through preconditioning. The aim of the present study is to test the hypothesis that preconditioning with pentylenetetrazole (PTZ) may have protective effect against seizure induced by i.v. infusion of PTZ. Mice were preconditioned by low-dose administration of PTZ (25 mg/kg) for 5 consecutive days, and the threshold of seizure elicited by i.v. infusion of PTZ was measured. To investigate the possible role of nitric oxide, NOS inhibitor enzymes, including L-NG-nitro-L-arginine methyl ester hydrochloride (L-NAME) (10 mg/kg), aminoguanidine (AG) (50 mg/kg), 7-nitroindazole (7-NI) (15 mg/kg), and L-arginine (L-arg) (60 mg/kg), were administered concomitantly with PTZ in both acute and chronic phases. Determination of seizure threshold revealed significant enhancement after preconditioning with low dose of PTZ. While the protective effect of PTZ preconditioning was enhanced after the administration of L-arg, it was reversed following administration of L-NAME and 7NI, suggesting the involvement of nitric oxide pathway as an underlying mechanism of PTZ-induced preconditioning. Preconditioning with PTZ led to brain tolerance and adaptive response in animal model of PTZ-induced seizure. This effect is in part due to the involvement of nitric oxide pathway. |
| Databáze: | OpenAIRE |
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