Characterization of a novel styrylbenzimidazolium‐based dye and its application in the detection of biothiols
| Autor: | Aleksandra Grzelakowska, Jolanta Kolińska |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Biophysics
Quantum yield 02 engineering and technology Mass spectrometry 01 natural sciences HeLa chemistry.chemical_compound Humans Cysteine Spectroscopy Homocysteine Maleimide Fluorescent Dyes Detection limit biology 010401 analytical chemistry 021001 nanoscience & nanotechnology biology.organism_classification Glutathione Fluorescence 0104 chemical sciences Spectrometry Fluorescence chemistry Chemistry (miscellaneous) Proton NMR 0210 nano-technology HeLa Cells Nuclear chemistry |
| Zdroj: | Luminescence. 36:409-417 |
| ISSN: | 1522-7243 1522-7235 |
| DOI: | 10.1002/bio.3956 |
| Popis: | A novel styrylbenzimidazolium dye containing a maleimide group 5 was synthesized and characterized using proton nuclear magnetic resonance spectroscopy and mass spectrometry. The photophysical properties [ultraviolet-visible (UV-vis) light absorption and fluorescence spectra, fluorescence quantum yield, and fluorescence lifetime] were investigated. Spectroscopic characterization of the novel styrylbenzimidazolium-based dye under various conditions is presented and its usefulness to detect biothiols proved. The addition of biothiols [l-cysteine (l-Cys), l-homocysteine (l-Hcy), l-glutathione (l-GSH)] to compound 5 in phosphate buffer (0.1 M, pH 7.4) containing 10% CH3 CN induced a 15-28-fold enhancement in fluorescence intensity at 410 nm. The limits of detection of compound 5 for l-Cys, l-Hcy, and l-GSH were estimated as 0.114, 0.118, and 0.059 μM, respectively. Evaluation of the cytotoxicity of 5 using the PrestoBlue assay for HeLa cells was also determined. The examined compound revealed a slight cytotoxicity against HeLa cells under experimental conditions. |
| Databáze: | OpenAIRE |
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