QKI6B mRNA levels are upregulated in schizophrenia and predict GFAP expression
| Autor: | Bryn Farnsworth, Katarzyna J. Radomska, Bettina M. Zimmermann, Elena Jazin, Petronella Kettunen, Lina Emilsson |
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| Rok vydání: | 2017 |
| Předmět: |
Gene isoform
Adult Male Response element Gene Expression Prefrontal Cortex Real-Time Polymerase Chain Reaction 03 medical and health sciences Young Adult 0302 clinical medicine Downregulation and upregulation Glial Fibrillary Acidic Protein medicine Humans RNA Messenger Prefrontal cortex Molecular Biology Gene Aged Aged 80 and over Glial fibrillary acidic protein biology General Neuroscience RNA-Binding Proteins Middle Aged medicine.disease 030227 psychiatry Cell biology Oligodendroglia medicine.anatomical_structure Schizophrenia Astrocytes Multivariate Analysis biology.protein Linear Models Female Neurology (clinical) Psychology Neuroscience 030217 neurology & neurosurgery Developmental Biology Astrocyte |
| Zdroj: | Brain research. 1669 |
| ISSN: | 1872-6240 |
| Popis: | Schizophrenia is a highly heritable disorder with a heterogeneous symptomatology. Research increasingly indicates the importance of the crucial and often overlooked glial perturbations within schizophrenia. Within this study, we examined an isoform of quaking (a gene encoding an RNA-binding protein that is exclusively expressed in glial cells), known as QKI6B, and a prototypical astrocyte marker, glial fibrillary acidic protein (GFAP), postulated to be under the regulation of QKI. The expression levels of these genes were quantified across post-mortem brain samples from 55 schizophrenic individuals, and 55 healthy controls, using real-time PCR. We report, through an analysis of covariance (ANCOVA) model, an upregulation of both QKI6B, and GFAP in the prefrontal cortex of brain samples of schizophrenic individuals, as compared to control samples. Previous research has suggested that the QKI protein directly regulates the expression of several genes through interaction with a motif in the target's sequence, termed the Quaking Response Element (QRE). We therefore examined if QKI6B expression can predict the outcome of GFAP, and several oligodendrocyte-related genes, using a multiple linear regression approach. We found that QKI6B significantly predicts the expression of GFAP, but does not predict oligodendrocyte-related gene outcome, as previously seen with other QKI isoforms. |
| Databáze: | OpenAIRE |
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