Pseudomonas aeruginosa PAO1 Resistance to Zinc Pyrithione: Phenotypic Changes Suggest the Involvement of Efflux Pumps
| Autor: | I. S. I. Al-Adham, Philip J. Collier, Khalid Z. Matalka, Suzanne M. Abdel Malek |
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| Rok vydání: | 2009 |
| Předmět: |
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Imipenem Pyridines Biological Transport Active chemistry.chemical_element Microbial Sensitivity Tests Drug resistance Zinc Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Minimum inhibitory concentration Bacterial Proteins Drug Resistance Bacterial Organometallic Compounds medicine Uncoupling Agents Pseudomonas aeruginosa Chloramphenicol Membrane Transport Proteins General Medicine Anti-Bacterial Agents chemistry Efflux Bacterial outer membrane medicine.drug |
| Zdroj: | Current Microbiology. 59:95-100 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-009-9396-9 |
| Popis: | The aim of this study is to investigate the involvement of an efflux pump in the development of Pseudomonas aeruginosa resistance to zinc pyrithione (ZnPT). In the presence of efflux inhibitor carbonyl cyanide m-chlorophenyl-hydrazone (CCCP), the minimum inhibitory concentration of ZnPT for P. aeruginosa resistant cells is reduced significantly (p < 0.05). In addition, the concentration of ZnPT excluded by the resistant bacteria was reduced significantly (p < 0.01). However, the above reductions did not reach the levels measured for P. aeruginosa PAO1 sensitive strain. Furthermore, such changes in P. aeruginosa resistant cells were correlated with the overexpression of outer membrane proteins, reduced sensitivity toward imipenem (p < 0.01) and increased sensitivity toward sulphatriad and chloramphenicol (p < 0.05). In a continuation to a previous study, we conclude that P. aeruginosa resistance to ZnPT is multifactorial and involves induced efflux systems. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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