Development of a High-Throughput Cytometric Screen to Identify Anti-Wolbachia Compounds: The Power of Public–Private Partnership
| Autor: | Helen Plant, Kirsty Rich, Darren Plant, Paul Harper, Catherine Bardelle, Louise Ford, Eileen McCall, Barton E. Slatko, Lindsey J. Cantin, Matthew Collier, Bo Wu, Mark J. Taylor, Mark Wigglesworth, Stephen A. Ward, Kelly L. Johnston, David Murray, Rachel H. Clare, Roger Clark |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Phenotypic screening Cell Culture Techniques Computational biology Biology high-throughput screening 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences Elephantiasis Filarial Drug Discovery medicine Humans neglected tropical diseases lymphatic filariasis Lymphatic filariasis Original Research Image Cytometry Insect cell phenotypic screening onchocerciasis acumen biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease Anti-Bacterial Agents High-Throughput Screening Assays 0104 chemical sciences 010404 medicinal & biomolecular chemistry 030104 developmental biology Host-Pathogen Interactions Neglected tropical diseases Molecular Medicine Wolbachia Biotechnology |
| Zdroj: | Slas Discovery |
| ISSN: | 2472-5552 |
| DOI: | 10.1177/2472555219838341 |
| Popis: | The Anti- Wolbachia (A·WOL) consortium at the Liverpool School of Tropical Medicine (LSTM) has partnered with the Global High-Throughput Screening (HTS) Centre at AstraZeneca to create the first anthelmintic HTS for neglected tropical diseases (NTDs). The A·WOL consortium aims to identify novel macrofilaricidal drugs targeting the essential bacterial symbiont ( Wolbachia) of the filarial nematodes causing onchocerciasis and lymphatic filariasis. Working in collaboration, we have validated a robust high-throughput assay capable of identifying compounds that selectively kill Wolbachia over the host insect cell. We describe the development and validation process of this complex, phenotypic high-throughput assay and provide an overview of the primary outputs from screening the AstraZeneca library of 1.3 million compounds. |
| Databáze: | OpenAIRE |
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