Distinct Expression of Nonmuscle Myosin IIB in Pulmonary Arteries of Patients With Aortic Stenosis vs Insufficiency Undergoing a Ross Procedure
| Autor: | Laurence Lefebvre, Louis Villeneuve, Alexander Emmott, Vanessa Hertig, Ismail El-Hamamsy, Richard L. Leask, Angelino Calderone, Alexandre Bergeron |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Pathology medicine.medical_specialty Vascular smooth muscle medicine.medical_treatment Aortic Valve Insufficiency Myocytes Smooth Muscle Pulmonary Artery 030204 cardiovascular system & hematology Immunofluorescence 03 medical and health sciences 0302 clinical medicine Elastic Modulus Nonmuscle myosin medicine.artery medicine Humans 030212 general & internal medicine Cardiac skeleton Autografts Aorta Pulmonary Valve Nonmuscle Myosin Type IIB medicine.diagnostic_test biology business.industry Ross procedure Aortic Valve Stenosis Middle Aged medicine.disease Actins Echocardiography Doppler Biomechanical Phenomena Elastin Stenosis Pulmonary artery biology.protein Female Collagen Tunica Media Cardiology and Cardiovascular Medicine business |
| Zdroj: | Canadian Journal of Cardiology. 37:47-56 |
| ISSN: | 0828-282X |
| DOI: | 10.1016/j.cjca.2020.02.074 |
| Popis: | Clinical studies have revealed a greater risk of pulmonary autograft dilation after the Ross procedure in patients with preoperative aortic insufficiency (AI). The present study examined whether the morphologic, biomechanical, and cellular properties of the pulmonary artery (PA) from patients with AI were phenotypically different compared with patients diagnosed with aortic stenosis (AS).PA segments were harvested from patients undergoing the Ross procedure for AS (n = 16) and AI (n = 6). Preoperative aortic annulus was significantly larger (P0.05) in patients with AI (28.5 ± 1.8 mm) vs AS (22.8 ± 1.2 mm). Morphologic, biomechanical, and cellular phenotypes of the PA were analyzed.Collagen and elastin content in the media of the PA wall were similar in patients with AS and AI. Elastic modulus and energy loss of the PA were not significantly different between the groups. In the media of the PA, expression of a panel of vascular smooth muscle cell-specific proteins were similar in patients with AS and AI. In contrast, nonmuscle myosin IIB protein levels in the PA of AS patients were significantly higher compared with AI patients, and immunofluorescence identified staining in α-smooth muscle actin-positive vascular smooth muscle cells.Despite similar morphological and biomechanical properties, the disparate expression of nonmuscle myosin IIB protein distinguishes the PA of patients with AI from patients with AS. The biological role in vascular smooth muscle cells and the potential contribution of nonmuscle myosin IIB to pulmonary autograft dilation in a subset of AI patients after the Ross procedure remain to be determined. |
| Databáze: | OpenAIRE |
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