Anti-Apoptotic and Pro-Survival Effect of Alpinate Oxyphyllae Fructus (AOF) in a d-Galactose-Induced Aging Heart
| Autor: | Yu Lan Yeh, Hsin Nung Chang, Hung-Jen Lin, Vijaya Padma Viswanadha, Yung Ming Chang, Chih Yang Huang, Ray Jade Chen, Wei Wen Kuo, Hen-Hong Chang, Chin Chuan Tsai |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Aging lcsh:Chemistry Rats Sprague-Dawley Phosphatidylinositol 3-Kinases Sirtuin 1 Myocytes Cardiac Medicine Chinese Traditional lcsh:QH301-705.5 Spectroscopy chemistry.chemical_classification TUNEL assay Kinase apoptosis General Medicine Immunohistochemistry Computer Science Applications Echocardiography medicine.medical_specialty Blotting Western bcl-X Protein AOF Biology Article Catalysis Inorganic Chemistry SIRT1 03 medical and health sciences Zingiberaceae Internal medicine medicine Animals Physical and Theoretical Chemistry Molecular Biology Protein kinase B PI3K/AKT/mTOR pathway Reactive oxygen species Plant Extracts Organic Chemistry Galactose Rats 030104 developmental biology Endocrinology lcsh:Biology (General) lcsh:QD1-999 Terminal deoxynucleotidyl transferase chemistry Apoptosis Fruit biology.protein d-galactose-induced aging Reactive Oxygen Species Proto-Oncogene Proteins c-akt |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 17, Iss 4, p 466 (2016) International Journal of Molecular Sciences; Volume 17; Issue 4; Pages: 466 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms17040466 |
| Popis: | Aging, a natural biological/physiological phenomenon, is accelerated by reactive oxygen species (ROS) accumulation and identified by a progressive decrease in physiological function. Several studies have shown a positive relationship between aging and chronic heart failure (HF). Cardiac apoptosis was found in age-related diseases. We used a traditional Chinese medicine, Alpinate Oxyphyllae Fructus (AOF), to evaluate its effect on cardiac anti-apoptosis and pro-survival. Male eight-week-old Sprague–Dawley (SD) rats were segregated into five groups: normal control group (NC), d-Galactose-Induced aging group (Aging), and AOF of 50 (AL (AOF low)), 100 (AM (AOF medium)), 150 (AH (AOF high)) mg/kg/day. After eight weeks, hearts were measured by an Hematoxylin–Eosin (H&E) stain, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-assays and Western blotting. The experimental results show that the cardiomyocyte apoptotic pathway protein expression increased in the d-Galactose-Induced aging groups, with dose-dependent inhibition in the AOF treatment group (AL, AM, and AH). Moreover, the expression of the pro-survival p-Akt (protein kinase B (Akt)), Bcl-2 (B-cell lymphoma 2), anti-apoptotic protein (Bcl-xL) protein decreased significantly in the d-Galactose-induced aging group, with increased performance in the AOF treatment group with levels of p-IGFIR and p-PI3K (Phosphatidylinositol-3′ kinase (PI3K)) to increase by dosage and compensatory performance. On the other hand, the protein of the Sirtuin 1 (SIRT1) pathway expression decreased in the aging groups and showed improvement in the AOF treatment group. Our results suggest that AOF strongly works against ROS-induced aging heart problems. |
| Databáze: | OpenAIRE |
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