Sensitive and Specific New Enzyme-Linked Immunosorbent Assay for N-ERC/Mesothelin Increases its Potential as a Useful Serum Tumor Marker for Mesothelioma
| Autor: | Kazuhisa Takahashi, Masashi Kobayashi, Yuji Natori, Kazu Shiomi, Kouji Sonoue, Kazuya Miyashita, Yukinori Sakao, Masataka Hirabayashi, Akira Inoue, Tatsuya Segawa, Atsuko Ishida, Takao Moroboshi, Hideaki Miyamoto, Masahiro Maeda, Okio Hino, Yoshiaki Hagiwara, Hiroshi Izumi, Takashi Yoshiyama, Kimihiko Masuda |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Mesothelioma Oncology Cancer Research Pathology Lung Neoplasms endocrine system diseases Asbestosis Mice Cricetinae Histologic type Stage (cooking) Cells Cultured Aged 80 and over Membrane Glycoproteins biology Antibodies Monoclonal Middle Aged respiratory system medicine.anatomical_structure Mesothelin Female medicine.medical_specialty Pleural Neoplasms Blotting Western Enzyme-Linked Immunosorbent Assay CHO Cells GPI-Linked Proteins Sensitivity and Specificity Cricetulus Peritoneum Internal medicine Biomarkers Tumor medicine Animals Humans Aged Tumor marker business.industry Asbestos medicine.disease respiratory tract diseases Case-Control Studies Potential biomarkers biology.protein business |
| Zdroj: | Clinical Cancer Research. 14:1431-1437 |
| ISSN: | 1557-3265 1078-0432 |
| DOI: | 10.1158/1078-0432.ccr-07-1613 |
| Popis: | Background: Because mesothelioma initially progresses on the surface of the pleura and peritoneum without forming masses, it has been difficult to diagnose at an early stage. It would be very useful to identify a tumor marker that could be used for screening to enable more diagnoses to be made at an early, treatable stage. Materials and Methods: We had previously identified N-ERC/mesothelin as a potential biomarker for mesothelioma. In the current work, we used a newly developed ELISA system to gain data on N-ERC/mesothelin levels in various clinical settings. A total of 102 healthy volunteers were recruited. In addition, 39 patients were diagnosed with mesothelioma, 53 patients were diagnosed with diseases that should be distinguished from mesothelioma, and 201 subjects were diagnosed with asbestos-related nonmalignant diseases (including simple exposure to asbestosis) who were treated at any of the cooperating hospitals were enrolled. Results: Serum N-ERC/mesothelin levels measured by a new ELISA system showed that the median values from patients with mesothelioma were extremely high compared with levels obtained from other patients. Analysis in terms of histologic type showed that serum levels of N-ERC/mesothelin were elevated in epithelioid type mesothelioma, especially. In four important models of clinical settings, the sensitivity and specificity of N-ERC/mesothelin were about 71% to 90% and 88% to 93%, respectively. Conclusion: N-ERC/mesothelin is a very promising tumor marker for mesothelioma, especially epithelioid mesothelioma. |
| Databáze: | OpenAIRE |
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