Molecular Phylogenetic Diversity and Biological Characterization of Diaporthe Species Associated with Leaf Spots of Camellia sinensis in Taiwan
Autor: | Jaturong Kumla, Medsaii Tanjira, Jian Yuan Wang, Nakarin Suwannarach, Abdallah M. Elgorban, Ratchadawan Cheewangkoon, Hiran A. Ariyawansa, Patchareeya Withee, Ichen Tsai, Shiou Ruei Lin |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine endophytes Plant Science Biology 01 natural sciences Article 03 medical and health sciences taxonomy Diaporthe Phylogenetics Botany Leaf spot pathogenicity Camellia sinensis Internal transcribed spacer Ecology Evolution Behavior and Systematics Ecology Spots Phylogenetic tree food and beverages 030108 mycology & parasitology biology.organism_classification Phylogenetic diversity ddc:580 QK1-989 foliar pathogens 010606 plant biology & botany |
Zdroj: | Plants Volume 10 Issue 7 Plants, Vol 10, Iss 1434, p 1434 (2021) |
ISSN: | 2223-7747 |
DOI: | 10.3390/plants10071434 |
Popis: | Camellia sinensis is one of the major crops grown in Taiwan and has been widely cultivated around the island. Tea leaves are prone to various fungal infections, and leaf spot is considered one of the major diseases in Taiwan tea fields. As part of a survey on fungal species causing leaf spots on tea leaves in Taiwan, 19 fungal strains morphologically similar to the genus Diaporthe were collected. ITS (internal transcribed spacer), tef1-α (translation elongation factor 1-α), tub2 (beta-tubulin), and cal (calmodulin) gene regions were used to construct phylogenetic trees and determine the evolutionary relationships among the collected strains. In total, six Diaporthe species, including one new species, Diaporthe hsinchuensis, were identified as linked with leaf spot of C. sinensis in Taiwan based on both phenotypic characters and phylogeny. These species were further characterized in terms of their pathogenicity, temperature, and pH requirements under laboratory conditions. Diaporthe tulliensis, D. passiflorae, and D. perseae were isolated from C. sinensis for the first time. Furthermore, pathogenicity tests revealed that, with wound inoculation, only D. hongkongensis was pathogenic on tea leaves. This investigation delivers the first assessment of Diaporthe taxa related to leaf spots on tea in Taiwan. |
Databáze: | OpenAIRE |
Externí odkaz: |