Suppressive effect of Aurantii Fructus Immaturus and Atractylodis Macrocephalae Rhizoma on glutamic acid-induced autophagy of interstitial cells of Cajal
| Autor: | Shuai Yan, Wu Bensheng, Yinzi Yue, Xiaopeng Wang, Mingming Sun |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0211 other engineering and technologies
Glutamic Acid Apoptosis 02 engineering and technology Flow cytometry Phosphatidylinositol 3-Kinases 03 medical and health sciences symbols.namesake 0302 clinical medicine 021105 building & construction Autophagy medicine Animals Protein kinase B PI3K/AKT/mTOR pathway medicine.diagnostic_test Chemistry TOR Serine-Threonine Kinases Atractylodes General Medicine Interstitial Cells of Cajal Molecular biology Rats 030205 complementary & alternative medicine Interstitial cell of Cajal Oncogene Protein v-akt Blot Real-time polymerase chain reaction symbols Microtubule-Associated Proteins Rhizome Drugs Chinese Herbal Signal Transduction |
| Zdroj: | Journal of Integrative Medicine. 18:334-343 |
| ISSN: | 2095-4964 |
| DOI: | 10.1016/j.joim.2020.04.005 |
| Popis: | To investigate the effects of Aurantii Fructus Immaturus (Zhishi, ZS) and Atractylodis Macrocephalae Rhizoma (Baizhu, BZ)-containing serum on glutamate-induced autophagy in rat colonic interstitial cells of Cajal (ICCs) and to analyze the underlying mechanism.Rat colonic ICCs cultured in vitro were identified by fluorescence and then stimulated with glutamic acid (5 mmol/L) for 24 h to establish a cell model of autophagy. The cells were then treated with different concentrations of ZSBZ-containing serum or rat serum. The viability of the ICCs was detected with cell counting kit-8 assays, and cell apoptosis rates were examined with flow cytometry. The ultrastructure and autophagosomes in the ICCs were observed using transmission electron microscopy. The effects of ZSBZ-containing serum on apoptosis-associated mediators were assessed by Western blotting and real-time quantitative polymerase chain reaction. In addition, microtubule-associated protein light chain 3 (LC3), p-phosphoinositide 3-kinase (p-PI3K), p-Akt and p-mammalian target of rapamycin (p-mTOR) expression was detected via Western blotting analysis.Compared to those in the model group, ICC viability and apoptosis rates were significantly increased by ZSBZ-containing serum (P 0.05). In addition, the expression levels of Beclin-1, LC3, p-PI3K, p-Akt and p-mTOR were significantly lower (P 0.05) and Bcl-2 expression was higher in the ZSBZ-containing serum treatment groups than in the model group (P 0.05).Our findings demonstrated that ZSBZ protects glutamic acid-stimulated ICCs, and this beneficial effect may be mediated by a reduction in autophagy via inhibition of the PI3K/Akt/mTOR pathway. |
| Databáze: | OpenAIRE |
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