A role for specific collagen motifs during wound healing and inflammatory response of fibroblasts in the teleost fish gilthead seabream
| Autor: | Dominique Bihan, Patricia Castillo-Briceño, Victoriano Mulero, Richard W. Farndale, Alfonsa García-Ayala, José Meseguer, Samir W. Hamaia, Michael Nilges |
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| Přispěvatelé: | Department of Cell Biology and Histology [Murcia], Universidad de Murcia, Department of Biochemistry [Cambridge], University of Cambridge [UK] (CAM), Département de Biologie structurale et Chimie - Department of Structural Biology and Chemistry, Institut Pasteur [Paris] (IP), We thank I Fuentes for her efficient technical assistance, the 'Servicio de Apoyo a la Investigación' of the University of Mur- cia for the cell culture and biomolecular analysis assistance, and Roche Applied Science (Spain) for lending the RTCA analyzers used in this work. This work was supported by the Spanish Ministry of Science and Innovation (grants AGL2008-04575-CO2-01 to A.G.-A. and BIO2008-01379 to V.M., and fellowship AP2005-5007 to P.C.- B.), Fundación Séneca, CARM (grant 04538/GERM/06 to A.G.-A.), and Fundación Marcelino Botín (grant to V.M.). Peptide synthesis was supported by grants from the Wellcome Trust and Medical Research Council, UK (R.W.F.)., University of Murcia, Institut Pasteur [Paris] |
| Rok vydání: | 2011 |
| Předmět: |
MESH: Antigens
CD29 MESH: Inflammation Amino Acid Motifs Interleukin-1beta MESH: Sea Bream Integrin MESH: Amino Acid Sequence MESH: Receptors Pattern Recognition Extracellular matrix MESH: Amino Acid Motifs MESH: Structure-Activity Relationship 0302 clinical medicine MESH: Interleukin-1beta Sparus aurata MESH: Collagen MESH: Magnesium MESH: Animals Magnesium Cyclooxygenase-2 MESH: Static Electricity Regulation of gene expression 0303 health sciences biology MESH: Peptides Protein Stability Integrin beta1 MESH: Gene Expression Regulation [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] Interleukin-1β Collagen motifs Respiratory burst Biochemistry Receptors Pattern Recognition 030220 oncology & carcinogenesis Adhesion Collagen MESH: Cyclooxygenase 2 Molecular Sequence Data Static Electricity Immunology Wound healing Article MESH: Cell Adhesion Proinflammatory cytokine Structure-Activity Relationship 03 medical and health sciences MESH: Protein Stability Cell Adhesion Animals Humans Amino Acid Sequence RNA Messenger Cell adhesion Molecular Biology MESH: RNA Messenger 030304 developmental biology Inflammation MESH: Humans MESH: Molecular Sequence Data Fibroblasts Sea Bream In vitro MESH: Wound Healing Gene Expression Regulation MESH: Fibroblasts Cyclooxygenase 2 biology.protein Peptides Teleost fish Interleukin-1 |
| Zdroj: | Molecular Immunology Molecular Immunology, 2011, 48, pp.826-34. ⟨10.1016/j.molimm.2010.12.004⟩ Molecular Immunology, Elsevier, 2011, 48, pp.826-34. ⟨10.1016/j.molimm.2010.12.004⟩ |
| ISSN: | 0161-5890 |
| DOI: | 10.1016/j.molimm.2010.12.004 |
| Popis: | International audience; Specific sites and sequences in collagen to which cells can attach, either directly or through protein intermediaries, were identified using Toolkits of 63-amino acid triple-helical peptides and specific shorter GXX'GEX″ motifs, which have different intrinsic affinity for integrins that mediate cell adhesion and migration. We have previously reported that collagen type I (COL-I) was able to prime in vitro the respiratory burst and induce a specific set of immune- and extracellular matrix-related molecules in phagocytes of the teleost fish gilthead seabream (Sparus aurata L.). It was also suggested that COL-I would provide an intermediate signal during the early inflammatory response in gilthead seabream. Since fibroblasts are highly involved in the initiation of wound repair and regeneration processes, in the present study SAF-1 cells (gilthead seabream fibroblasts) were used to identify the binding motifs in collagen by end-point and real-time cell adhesion assays using the collagen peptides and Toolkits. We identified the collagen motifs involved in the early magnesium-dependent adhesion of these cells. Furthermore, we found that peptides containing the GFOGER and GLOGEN motifs (where O is hydroxyproline) present high affinity for SAF-1 adhesion, expressed as both cell number and surface covering, while in cell suspensions, these motifs were also able to induce the expression of the genes encoding the proinflammatory molecules interleukin-1β and cyclooxygenase-2. These data suggest that specific collagen motifs are involved in the regulation of the inflammatory and healing responses of teleost fish. |
| Databáze: | OpenAIRE |
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