Lymphocyte subpopulations of intestinal mucosa in inflammatory bowel disease
| Autor: | W.L. Beeken, S.St. Andre-Ukena, O.E. Eade, C. Moulton, B.R. MacPherson |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Rosette Formation T-Lymphocytes Cell Ficoll Fc receptor Receptors Antigen B-Cell Biology Inflammatory bowel disease Dithiothreitol Leukocyte Count chemistry.chemical_compound Crohn Disease Intestinal mucosa medicine Humans Lymphocytes Intestinal Mucosa Aged B-Lymphocytes Gastroenterology Middle Aged medicine.disease digestive system diseases Staining medicine.anatomical_structure chemistry Immunology Collagenase biology.protein Colitis Ulcerative Female Research Article medicine.drug |
| Zdroj: | Gut. 21:675-682 |
| ISSN: | 0017-5749 |
| Popis: | Lymphocyte subpopulations in peripheral blood (PBL) and intestinal mucosa (IML) of 10 patients with inflammatory bowel disease (IBD) were compared with those of 11 non-IBD controls. PBL were separated on Ficoll/hypaque gradients, and IML were isolated by incubation in dithiothreitol, EDTA, and collagenase. These methods yielded cells of good viability and with intact HLA A and B-antigens. T-cells, identified by neuraminidase-treated sheep RBC rosettes and non-specific esterase staining, comprised approximately 91% of the IML from normal mucosa of all groups. B-cells, identified by erythrocyte-antibody-complement rosettes and surface immunoglobulins, were only 7% of these IML populations. Cell yields were two-fold or more greater from abnormal IBD mucosa, with T-cells ranging from 55 to 95% and B-cells from 2 to 36%. The percentage of Fc receptor bearing cells was low in all specimens. By these methods, T-lymphocytes predominated in intestinal mucosa of both IBD and non-IBD patients, but there is marked increase in the percentage of B-cells isolated from abnormal mucosa in IBD. |
| Databáze: | OpenAIRE |
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