Collaborative Study of an Indirect Enzymatic Method for the Simultaneous Analysis of 3-MCPD, 2-MCPD, and Glycidyl Esters in Edible Oils
| Autor: | Yomi Watanabe, Kumiko Yamazaki, Tadashi Kitta, Takashi Miyashita, Yukinari Takahashi, Takashi Sano, Kinuko Miyazaki, Hiroshi Yada, Kazuo Koyama, Hidenori Nohara, Toru Nezu, Hideji Taniguchi, Hirotsugu Kido, Kousuke Abe, Yoshitsugu Egawa |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Glycerol
Rapeseed General Chemical Engineering alpha-Chlorohydrin chemistry.chemical_compound Hydrolysis 0404 agricultural biotechnology 3-MCPD Plant Oils Phenylboronic acid Lipase Candida chemistry.chemical_classification Chromatography biology Bran Glycidol Fatty acid 04 agricultural and veterinary sciences General Medicine General Chemistry 040401 food science chemistry biology.protein Epoxy Compounds Plants Edible |
| Zdroj: | SC10201706270011 |
| ISSN: | 1345-8957 |
| DOI: | 10.5650/jos.ess16021 |
| Popis: | A collaborative study was conducted to evaluate an indirect enzymatic method for the analysis of fatty acid esters of 3-monochloro-1,2-propanediol (3-MCPD), 2-monochloro-1,3-propanediol (2-MCPD), and glycidol (Gly) in edible oils and fats. The method is characterized by the use of Candida rugosa lipase, which hydrolyzes the esters at room temperature in 30 min. Hydrolysis and bromination steps convert esters of 3-MCPD, 2-MCPD, and glycidol to free 3-MCPD, 2-MCPD, and 3-monobromo-1,2-propanediol, respectively, which are then derivatized with phenylboronic acid, and analyzed by gas chromatography-mass spectrometry. In a collaborative study involving 13 laboratories, liquid palm, solid palm, rapeseed, and rice bran oils spiked with 0.5-4.4 mg/kg of esters of 3-MCPD, 2-MCPD, and Gly were analyzed in duplicate. The repeatability (RSDr) were < 5% for five liquid oil samples and 8% for a solid oil sample. The reproducibility (RSDR) ranged from 5% to 18% for all oil samples. These RSDR values were considered satisfactory because the Horwitz ratios were ≤ 1.3% for all three analytes in all oil samples. This method is applicable to the quantification of 3-MCPD, 2-MCPD, and Gly esters in edible oils. |
| Databáze: | OpenAIRE |
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