Altered phenotype in peripheral blood and tumor-associated NK cells from colorectal cancer patients
| Autor: | Juan Martín Arriaga, Eduardo Huertas, José Mordoh, Luisina Inés Bruno, Alejandro Pairola, Estrella Mariel Levy, Maria Paula Roberti, Michele Bianchini, Fernando Sanchez Loria, Mora Amat, Yamila S. Rocca, María Betina Pampena |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male CIENCIAS MÉDICAS Y DE LA SALUD Immunology Cetuximab Medicina Clínica Biology Microbiology Cell Degranulation Oncología Immunophenotyping Interleukin 21 Interferon-gamma Immune system Lymphocytes Tumor-Infiltrating NK-92 purl.org/becyt/ford/3.2 [https] medicine Humans Interferon gamma Adcc Molecular Biology Cells Cultured Aged Colorectal Cancer Aged 80 and over Lymphokine-activated killer cell Blood Cells Janus kinase 3 Immune Supresion Cell Biology Middle Aged Natural Killer Cells Killer Cells Natural Infectious Diseases Cell Transformation Neoplastic Interleukin 12 Receptors Natural Killer Cell purl.org/becyt/ford/3 [https] Female Colorectal Neoplasms medicine.drug |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1753-4267 |
| Popis: | Despite NK cells being originally identified because of their ability to kill tumor cells in vitro, only limited information is available on NK cells infiltration of malignant tumors, especially in humans. NK cells infiltrating human colorectal carcinomas (CRCs) were analyzed to identify their potential protective role in an antitumor immune response. The expression and function of relevant molecules were analyzed from different sources, comparing tumor-associated NK cells (TANKs) with autologous peripheral blood NK cells (PB-NKs) from CRC patients-the latter in comparison with PB-NKs from normal donors. TANKs displayed a profound alteration of their phenotype with a drastic reduction of NK cell receptor expression. Co-culture experiments showed that CRC cells produce modulation in NK phenotype and functionality. Moreover, PB-NKs from CRC patients also exhibited an altered phenotype and profound defects in the ability to activate degranulation and IFN-γ production. For the first time, TANK and PB-NK cells from CRC patients have been characterized. It is shown that they are not capable of producing relevant cytokines and degranulate. Taken together, our results suggest that NK cells from CRC patients present alterations of phenotype and function therefore supporting the progression of cancer. Fil: Rocca, Yamila Sol. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina Fil: Roberti, Maria Paula. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina Fil: Arriaga, Juan Martín. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina Fil: Amat, Mora. Instituto MÉdico Especializado Alexander Fleming; Fil: Bruno, Luisina. Instituto MÉdico Especializado Alexander Fleming; Fil: Pampena, María Betina. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina Fil: Huertas, Eduardo. Instituto Médico Especializado Alexander Fleming; Fil: Sánchez Loria, Fernando. Instituto Médico Especializado Alexander Fleming; Fil: Pairola, Alejandro. Instituto Médico Especializado Alexander Fleming; Fil: Bianchini, Michele. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina Fil: Mordoh, Jose. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina Fil: Levy, Estrella Mariel. Fundacion Cancer. Centro de Investigaciones Oncologicas; Argentina |
| Databáze: | OpenAIRE |
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