In vivo testing of an injectable matrix gel for the treatment of shoulder cuff muscle fatty degeneration
| Autor: | Grady Dunlap, John T. Kim, Shahryar Ahmadi, Jeffrey C. Wolchok, Tai Huynh |
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| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Tenodesis Inflammation Osteoarthritis Injections Intramuscular Article Rotator Cuff Injuries Rats Sprague-Dawley Extracellular matrix Rotator Cuff 03 medical and health sciences 0302 clinical medicine Fibrosis Internal medicine Materials Testing medicine Animals Orthopedics and Sports Medicine Muscle Skeletal 030222 orthopedics business.industry Cardiac muscle Skeletal muscle 030229 sport sciences General Medicine medicine.disease Muscle atrophy Extracellular Matrix Rats Tendon Disease Models Animal Muscular Atrophy medicine.anatomical_structure Endocrinology Adipose Tissue Surgery Rabbits medicine.symptom business Gels |
| Zdroj: | J Shoulder Elbow Surg |
| ISSN: | 1058-2746 |
| Popis: | Introduction Extracellular matrix (ECM) gels have shown efficacy for the treatment of damaged tissues, most notably cardiac muscle. We hypothesized that the ECM gel prepared from skeletal muscle could be used as a treatment strategy for fatty shoulder cuff muscle degeneration. Methods We conducted experiments to (1) evaluate host biocompatibility to ECM gel injection using a rat model and (2) examine the effect of ECM gel injection on muscle recovery after delayed repair of a released supraspinatus (SSP) tendon using a rabbit model. Results The host biocompatibility to the ECM gel was characterized by a transient rise (first 2 weeks only) in several genes associated with macrophage infiltration, matrix deposition, and inflammatory cytokine production. By 8 weeks all genes had returned to baseline levels and no evidence of fibrosis or chronic inflammation was observed from histology. When gel injection was combined with SSP tendon repair, we observed a significant reduction (7%) in SSP muscle atrophy (24 + 3% reduction from uninjured) when compared with treatment with tendon repair only (31 + 7% reduction). Although fatty degeneration was elevated in both treatment groups, fat content trended lower (2%) in response to combined tendon repair and intramuscular ECM injection (4.1 + 2.1%) when compared with tendon repair only (6.1 + 2.9%). Transcriptome analysis revealed adipogenesis and osteoarthritis pathway activation in the repair only group. These key pathways were abrogated in response to treatment using combined repair plus gel. Discussion The findings suggest that ECM injection had a modest but positive effect on muscle mass, fatty degeneration, and key cellular signaling pathways. |
| Databáze: | OpenAIRE |
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