Effect of tenascin and fibronectin on the migration of human corneal fibroblasts
| Autor: | Gerald Schmidinger, Birgit Lackner, S. Kaminski, Georg Hanselmayer, Irene Ruhswurm, Stefan Pieh, Christian Skorpik |
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| Rok vydání: | 2003 |
| Předmět: |
Stromal cell
Tenascin Fibroblast growth factor Collagen Type I Cornea Cell Movement Cell Adhesion medicine Humans Vimentin Fibroblast Cells Cultured Migration Assay Dose-Response Relationship Drug biology Chemistry Fibroblasts Transforming Growth Factor alpha Molecular biology eye diseases Sensory Systems Fibronectins Fibronectin Ophthalmology medicine.anatomical_structure Immunology biology.protein Fibroblast Growth Factor 2 Surgery sense organs Wound healing Transforming growth factor |
| Zdroj: | Journal of Cataract and Refractive Surgery. 29:354-360 |
| ISSN: | 0886-3350 |
| DOI: | 10.1016/s0886-3350(02)01609-7 |
| Popis: | To investigate the effect of fibronectin and tenascin on the migration of corneal fibroblasts.Department of Ophthalmology, University of Vienna, Medical School, Vienna, Austria.Using human corneal fibroblasts, a monolayer migration assay was performed to measure corneal fibroblast movement. The migration on collagen I, fibronectin, and tenascin with and without transforming growth factor (TGF)-alpha/fibroblast growth factor (FGF)-2 stimulation and the effect of soluble tenascin and fibronectin on corneal fibroblast migration on collagen-I-coated wells were investigated.The cytokines TGF-alpha and FGF-2 stimulated migrational activity of corneal stromal cells in a dose-dependent manner, reaching the maximum effect at 100 ng/mL and 10 ng/mL, respectively. The migration of corneal fibroblasts on fibronectin was significantly higher (P.05) than the migration on collagen I. Transforming growth factor-alpha and FGF-2 increased radial cell displacement independent of the provided matrix composition. Tenascin had a negative effect on corneal fibroblast adhesion/migration in this in vitro model.Fibronectin and tenascin influenced corneal fibroblast migration and adhesion, respectively, and may play a role in stromal cell movement during wound healing. The cytokines TGF-alpha and FGF-2 had an additive effect on corneal fibroblast migration on a fibronectin matrix. |
| Databáze: | OpenAIRE |
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