Genetic Architecture of Flooding Tolerance in the Dry Bean Middle-American Diversity Panel
| Autor: | Phillip E. McClean, Katelynn Walter, Samira MafiMoghaddam, Sujan Mamidi, Juan M. Osorno, Stephan Schroder, Daniel Restrepo-Montoya, Rian Lee, Ali Soltani |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germplasm abiotic stress Plant Science lcsh:Plant culture 01 natural sciences Crop 03 medical and health sciences flooding parasitic diseases GWAS lcsh:SB1-1110 Cultivar Original Research common bean biology Abiotic stress Flooding (psychology) fungi food and beverages anoxia biology.organism_classification Genetic architecture waterlogging 030104 developmental biology Agronomy Seedling Phaseolus 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science, Vol 8 (2017) Frontiers in Plant Science |
| DOI: | 10.3389/fpls.2017.01183/full |
| Popis: | Flooding is a devastating abiotic stress that endangers crop production in the twenty-first century. Because of the severe susceptibility of common bean (Phaseolus vulgaris L.) to flooding, an understanding of the genetic architecture and physiological responses of this crop will set the stage for further improvement. However, challenging phenotyping methods hinder a large-scale genetic study of flooding tolerance in common bean and other economically important crops. A greenhouse phenotyping protocol was developed to evaluate the flooding conditions at early stages. The Middle-American diversity panel (n = 272) of common bean was developed to capture most of the diversity exits in North American germplasm. This panel was evaluated for seven traits under both flooded and non-flooded conditions at two early developmental stages. A subset of contrasting genotypes was further evaluated in the field to assess the relationship between greenhouse and field data under flooding condition. A genome-wide association study using ~150 K SNPs was performed to discover genomic regions associated with multiple physiological responses. The results indicate a significant strong correlation (r > 0.77) between greenhouse and field data, highlighting the reliability of greenhouse phenotyping method. Black and small red beans were the least affected by excess water at germination stage. At the seedling stage, pinto and great northern genotypes were the most tolerant. Root weight reduction due to flooding was greatest in pink and small red cultivars. Flooding reduced the chlorophyll content to the greatest extent in the navy bean cultivars compared with other market classes. Races of Durango/Jalisco and Mesoamerica were separated by both genotypic and phenotypic data indicating the potential effect of eco-geographical variations. Furthermore, several loci were identified that potentially represent the antagonistic pleiotropy. The GWAS analysis revealed peaks at Pv08/1.6 Mb and Pv02/41 Mb that are associated with root weight and germination rate, respectively. These regions are syntenic with two QTL reported in soybean (Glycine max L.) that contribute to flooding tolerance, suggesting a conserved evolutionary pathway involved in flooding tolerance for these related legumes. |
| Databáze: | OpenAIRE |
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