Ribosomal Protein uL11 as a Regulator of Metabolic Circuits Related to Aging and Cell Cycle
| Autor: | Marek Tchórzewski, Mateusz Molon, Eliza Molestak, Monika Kula-Maximenko, Przemysław Grela |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Protein moonlighting
Ribosomal Proteins Saccharomyces cerevisiae Proteins Time Factors Transcription Genetic media_common.quotation_subject Mutant Regulator translation Saccharomyces cerevisiae Biology Spectrum Analysis Raman Ribosome Article Ribosomal protein Cell Wall Gene Expression Regulation Fungal Protein biosynthesis Protein Isoforms Amino Acid Sequence paralogs lcsh:QH301-705.5 media_common Principal Component Analysis Cell Cycle aging Longevity uL11 General Medicine Cell biology Oxidative Stress Gene Ontology Phenotype cell-cycle ribosome lcsh:Biology (General) Polyribosomes Protein Biosynthesis Mutation Vacuoles hypertrophy Function (biology) Gene Deletion Metabolic Networks and Pathways lifespan |
| Zdroj: | Cells, Vol 9, Iss 1745, p 1745 (2020) Cells Volume 9 Issue 7 |
| ISSN: | 2073-4409 |
| Popis: | Aging is a biological phenomenon common to all living organisms. It is thought that the rate of aging is influenced by diverse factors, in many cases related to the control of energy metabolism, i.e., the so-called pro-longevity effects of starvation. Translation, regarded as the main energy consumption process, lies at the center of interest, as it has a significant impact on the longevity phenomenon. It has been shown that perturbations in the translational apparatus may lead to a lower rate of aging. Therefore, the main aim of this study was to investigate aging in relation to the protein biosynthesis circuit, taking into account the uL11 ribosomal protein as a vital ribosomal element. To this end, we used set of yeast mutants with deleted single uL11A or uL11B genes and a double disruptant uL11AB mutant. We applied an integrated approach analyzing a broad range of biological parameters of yeast mutant cells, especially the longevity phenomenon, supplemented with biochemical and high throughput transcriptomic and metobolomic approaches. The analysis showed that the longevity phenomenon is not fully related to the commonly considered energy restriction effect, thus the slow-down of translation does not represent the sole source of aging. Additionally, we showed that uL11 can be classified as a moonlighting protein with extra-ribosomal function having cell-cycle regulatory potential. |
| Databáze: | OpenAIRE |
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