Impact of a new sampling buffer on faecal haemoglobin stability in a colorectal cancer screening programme by the faecal immunochemical test
| Autor: | Pietro P Di Dia, Guido Castiglione, Marco Zappa, Leonardo Ventura, Stefano Rapi, Beatrice Mallardi, Grazia Grazzini, Tiziana Rubeca, Filippo Cellai, Paola Mantellini |
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| Rok vydání: | 2016 |
| Předmět: |
Cancer Research
medicine.medical_specialty Epidemiology Buffers Specimen Handling Screening programme 03 medical and health sciences Feces Hemoglobins 0302 clinical medicine Animal science medicine Humans Mass Screening Sampling (medicine) Early Detection of Cancer business.industry Immunochemistry Significant difference Public Health Environmental and Occupational Health Clinical performance Prognosis Sample stability Surgery Oncology Specimen collection Colorectal cancer screening 030220 oncology & carcinogenesis 030211 gastroenterology & hepatology business Colorectal Neoplasms |
| Zdroj: | European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP). 26(4) |
| ISSN: | 1473-5709 |
| Popis: | Haemoglobin (Hb) stability in faecal samples is an important issue in colorectal cancer screening by the faecal immunochemical test (FIT) for Hb. This study evaluated the performance of the FIT-Hb (OC-Sensor Eiken) used in the Florence screening programme by comparing two different formulations of the buffer, both in an analytical and in a clinical setting. In the laboratory simulation, six faecal pools (three in each buffer type) were stored at different temperatures and analysed eight times in 10 replicates over 21 days. In the clinical setting, 7695 screenees returned two samples, using both the old and the new specimen collection device (SCD). In the laboratory simulation, 5 days from sample preparation with the buffer of the old SCD, the Hb concentration decreased by 40% at room temperature (25°C, range 22-28°C) and up to 60% at outside temperature (29°C, range 16-39°C), whereas with the new one, Hb concentration decreased by 10%. In the clinical setting, a higher mean Hb concentration with the new SCD compared with the old one was found (6.3 vs. 5.0 µg Hb/g faeces, respectively, P |
| Databáze: | OpenAIRE |
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