Natural Histogel-Based Bio-Scaffolds for Sustaining Angiogenesis in Beige Adipose Tissue

Autor: Eleni Dakou, Roberto Ronca, Stefania Mitola, Michela Corsini, Wandert Schaafsma, Maria Vliora, Paraskevi Sakellariou, Margherita Di Somma, Begoña Castro, Jef Vanparijs, Elisabetta Grillo, Cosetta Ravelli
Přispěvatelé: Faculty of Sciences and Bioengineering Sciences, Cell Genetics, Faculty of Physical Education and Physical Therapy, Human Physiology and Sports Physiotherapy Research Group, Physiotherapy, Human Physiology and Anatomy
Jazyk: angličtina
Předmět:
Zdroj: Cells
Cells, Vol 8, Iss 11, p 1457 (2019)
Volume 8
Issue 11
ISSN: 2073-4409
DOI: 10.3390/cells8111457
Popis: In the treatment of obesity and its related disorders, one of the measures adopted is weight reduction by controlling nutrition and increasing physical activity. A valid alternative to restore the physiological function of the human body could be the increase of energy consumption by inducing the browning of adipose tissue. To this purpose, we tested the ability of Histogel, a natural mixture of glycosaminoglycans isolated from animal Wharton jelly, to sustain the differentiation of adipose derived mesenchymal cells (ADSCs) into brown-like cells expressing UCP-1. Differentiated cells show a higher energy metabolism compared to undifferentiated mesenchymal cells. Furthermore, Histogel acts as a pro-angiogenic matrix, induces endothelial cell proliferation and sprouting in a three-dimensional gel in vitro, and stimulates neovascularization when applied in vivo on top of the chicken embryo chorioallantoic membrane or injected subcutaneously in mice. In addition to the pro-angiogenic activity of Histogel, also the ADSC derived beige cells contribute to activating endothelial cells. These data led us to propose Histogel as a promising scaffold for the modulation of the thermogenic behavior of adipose tissue. Indeed, Histogel simultaneously supports the acquisition of brown tissue markers and activates the vasculature process necessary for the correct function of the thermogenic tissue. Thus, Histogel represents a valid candidate for the development of bioscaffolds to increase the amount of brown adipose tissue in patients with metabolic disorders.
Databáze: OpenAIRE
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