A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device
| Autor: | Yong Wang, Tricia A. Harvat, David T. Eddington, Jose Oberholzer, Dongyoung Lee, Adeola F. Adewola |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Fluorescence-lifetime imaging microscopy Materials science General Chemical Engineering Microfluidics 01 natural sciences General Biochemistry Genetics and Molecular Biology Islets of Langerhans Mice 03 medical and health sciences Internal medicine Insulin Secretion medicine Animals Insulin Insulin secretion Microscale chemistry 030304 developmental biology 0303 health sciences geography geography.geographical_feature_category Multi parametric General Immunology and Microbiology General Neuroscience 010401 analytical chemistry Microfluidic Analytical Techniques Islet 0104 chemical sciences Cellular Biology Endocrinology Calcium influx Biomedical engineering |
| Zdroj: | Journal of Visualized Experiments : JoVE |
| ISSN: | 1940-087X |
| DOI: | 10.3791/1649 |
| Popis: | A microfluidic islet perifusion device was developed for the assessment of dynamic insulin secretion of multiple islets and simultaneous fluorescence imaging of calcium influx and mitochondrial potential changes. The device consists of three layers: first layer contains an array of microscale wells (500 mum diameter and 150 mum depth) that help to immobilize the islets while exposed to flow and maximize the exposed surface area of the islets; the second layer contains a circular perifusion chamber (3 mm deep, 7 mm diameter); and the third layer contains an inlet-mixing channel that fans out before injection into the perifusion chamber (2 mm in width, 19 mm in length, and 500 mum in height) for optimizing the mixing efficiency prior to entering the perifusion chamber. The creation of various glucose gradients including a linear, bell shape, and square shapes also can be created in the microfluidic perifusion network and is demonstrated. |
| Databáze: | OpenAIRE |
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