Multistructural biomimetic substrates for controlled cellular differentiation
| Autor: | Thilak K. Mudalige, Ganesh K Kanarpardy, Mircea V. Diudea, Adrian Florea, Alexandru S. Biris, Horea Matei, Stefana Balici, Carmen Mihaela Mihu, Anamaria Orza, Olga Soritau |
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| Rok vydání: | 2014 |
| Předmět: |
Cell signaling
Materials science Cell Survival Surface Properties Cellular differentiation Cell Culture Techniques Metal Nanoparticles Biocompatible Materials Bioengineering Nanotechnology Microscopy Atomic Force Regenerative Medicine Regenerative medicine Extracellular matrix Tissue engineering Biomimetics Laminin Humans Regeneration Myocytes Cardiac General Materials Science Electrical and Electronic Engineering Neurons Tissue Engineering Tissue Scaffolds biology Photoelectron Spectroscopy Stem Cells Mechanical Engineering Regeneration (biology) Cell Differentiation General Chemistry Immunohistochemistry Extracellular Matrix Cell biology Phenotype Mechanics of Materials biology.protein Collagen Stem cell Signal Transduction |
| Zdroj: | Nanotechnology. 25:065102 |
| ISSN: | 1361-6528 0957-4484 |
| Popis: | Multidimensional scaffolds are considered to be ideal candidates for regenerative medicine and tissue engineering based on their potential to provide an excellent microenvironment and direct the fate of the cultured cells. More recently, the use of stem cells in medicine has opened a new technological opportunity for controlled tissue formation. However, the mechanism through which the substrate directs the differentiation of stem cells is still rather unclear. Data concerning its specific surface chemistry, topology, and its signaling ability need to be further understood and analyzed. In our study, atomic force microscopy was used to study the stiffness, roughness, and topology of the collagen (Coll) and metallized collagen (MC) substrates, proposed as an excellent substrate for regenerative medicine. The importance of signaling molecules was studied by constructing a new hybrid signaling substrate that contains both collagen and laminin extracellular matrix (ECM) proteins. The cellular response-such as attachment capability, proliferation and cardiac and neuronal phenotype expression on the metallized and non-metallized hybrid substrates (collagen + laminin)-was studied using MTT viability assay and immunohistochemistry studies. Our findings indicate that such hybrid materials could play an important role in the regeneration of complex tissues. |
| Databáze: | OpenAIRE |
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