Evaluation of a new rapid diagnostic test for the detection of influenza and RSV
| Autor: | Joaquín R. Otero, Lola Folgueira, Sara Gomez, Carmen Vera, C. Prieto |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) viruses 030106 microbiology Acute respiratory disease Respiratory Syncytial Virus Infections Sensitivity and Specificity Rapid detection Virus Cell Line 03 medical and health sciences 0302 clinical medicine Influenza Human Humans Medicine 030212 general & internal medicine Retrospective Studies Rapid diagnostic test Diagnostic Tests Routine business.industry virus diseases Influenza a Gold standard (test) Virology Respiratory Syncytial Viruses Influenza B virus Breath Tests Influenza A virus Immunology business |
| Zdroj: | Enfermedades Infecciosas y Microbiología Clínica. 34:298-302 |
| ISSN: | 0213-005X |
| DOI: | 10.1016/j.eimc.2015.05.007 |
| Popis: | Introduction Influenza viruses and respiratory syncytial virus (RSV) can cause an acute respiratory disease that occurs seasonally in epidemic waves. This retrospective study was conducted to evaluate the Sofia® Influenza A + B and the Sofia® RSV fluorescence immunoassays (FIAs), two novel rapid detection tests (RDTs) for influenza A and B and RSV. Methods Two hundred and nine breath samples were selected from patients with respiratory symptoms determined to be positive/negative for influenza A, influenza B or RSV using one of the reference diagnostic techniques, cell culture and/or RT-PCR (Simplexa™Flu A/B & RSV). The Sofia Influenza A + B FIA was tested on 123 samples (63 from children and 60 from adults) and the Sofia RSV FIA was tested on 86 pediatric samples. Sensitivity and specificity values of both assays were calculated assuming the reference techniques as the gold standard. Results Sensitivity and specificity values for the Sofia Influenza A + B FIA were 73.1% and 97.8%, respectively. Sensitivity and specificity values for the Sofia RSV FIA were 87.5% and 86.7%, respectively. Conclusion The sensitivity results obtained for the two assays were considerably higher than those reported for other RDTs. In conclusion, the Sofia Influenza A + B and the Sofia RSV FIAs are appropriate tools for the rapid diagnosis of these viruses. |
| Databáze: | OpenAIRE |
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