Experimental model for acute kidney injury caused by uropathogenic Escherichia coli
Autor: | Anna Malska-Woźniak, Agnieszka Baranowska, Beata Skowron, Grażyna Więcek, Magdalena Strus, Jolanta Kaszuba-Zwoińska, Piotr B Heczko |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
Microbiology (medical)
medicine.medical_specialty Fractional excretion of sodium Urinary system 030232 urology & nephrology Urology experimental model Renal function lcsh:Medicine urologic and male genital diseases Excretion 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Escherichia coli Animals Uropathogenic Escherichia coli acute kidney injury (AKI) Rats Wistar Escherichia coli Infections Creatinine Kidney Pyelonephritis business.industry lcsh:R Acute kidney injury 030208 emergency & critical care medicine Acute Kidney Injury medicine.disease female genital diseases and pregnancy complications Rats Disease Models Animal Infectious Diseases medicine.anatomical_structure chemistry Urinary Tract Infections Rat Female business Kidney disease |
Zdroj: | Postępy Higieny i Medycyny Doświadczalnej, Vol 71, Iss 1, Pp 520-529 (2017) |
ISSN: | 1732-2693 0032-5449 |
Popis: | Introduction: Acute kidney injury (AKI) is the rapid deterioration of renal function, diagnosed on the basis of an increase in serum creatinine and abnormal urinary parameters. AKI is associated with increased risk of mortality or chronic kidney disease (CKD).The aim of the study was to develop an experimental model for AKI resulting from Escherichia coli-induced pyelonephritis. E. coli was isolated from a patient with clinical symptoms of urinary tract infection (UTI).Material/Methods: The study included three groups of female Wistar rats (groups 1, 2 and 3), in which pyelonephritis was induced by transurethral inoculation with highly virulent E. coli (105, 107 and 109 cfu/ml, respectively). Urine and blood samples for analysis were obtained prior to the inoculation (day 0), as well as 7, 14 and 21 days thereafter.Results: Aside from a microbiological examination of urine samples, daily urine output, serum creatinine (CreaS), creatinine clearance (CrCl), interleukin 6 (IL-6), fractional excretion of sodium (FENa) and fractional excretion of urea (FEUrea) were determined. A histopathological examination of kidney and urinary bladder specimens was conducted as well. While UTI-related pyelonephritis developed irrespective of E. coli inoculum size, AKI was observed only following transurethral administration of E. coli at the intermediate and high dose, i.e. 107 and 109 cfu/ml, respectively (group 2 and 3). Discussion: An increase in CreaS and abnormal diuresis were accompanied by changes in parameters specific for various forms of AKI, i.e. FENa and FEUrea. Based on these changes, administration of E. coli at 107 cfu/ml was demonstrated to induce renal AKI, whereas inoculation with 109 cfu/ml seemed to cause not only ascending pyelonephritis, but perhaps also bacteremia and urosepsis (prerenal component of AKI). |
Databáze: | OpenAIRE |
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