Trifolium Repens Blocks Proliferation in Chronic Myelogenous Leukemia via the BCR-ABL/STAT5 Pathway

Autor: Angela Nebbioso, Fabrizio Merciai, Lucia Altucci, Vincenzo Carafa, Crescenzo Massaro, Pasquale Termolino, Federica Sarno, Pietro Campiglia, Giacomo Pepe
Přispěvatelé: Sarno, Federica, Pepe, Giacomo, Termolino, Pasquale, Carafa, Vincenzo, Massaro, Crescenzo, Merciai, Fabrizio, Campiglia, Pietro, Nebbioso, Angela, Altucci, Lucia
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Cells
Volume 9
Issue 2
Cells, Vol 9, Iss 2, p 379 (2020)
Cells (2020). doi:10.3390/cells9020379 (registering DOI)
info:cnr-pdr/source/autori:Federica Sarno, Giacomo Pepe, Pasquale Termolino, Vincenzo Carafa, Crescenzo Massaro, Fabrizio Merciati, Pietro Campiglia, Angela Nebbioso and Lucia Altucci/titolo:Trifolium Repens Blocks Proliferation in Chronic Myelogenous Leukemia via the BCR-ABL%2FSTAT5 Pathwa/doi:10.3390%2Fcells9020379 (registering DOI)/rivista:Cells/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume
ISSN: 2073-4409
DOI: 10.3390/cells9020379
Popis: Some species of clover are reported to have beneficial effects in human diseases. However, little is known about the activity of the forage plant Trifolium repens, or white clover, which has been recently found to exert a hepatoprotective action. Scientific interest is increasingly focused on identifying new drugs, especially natural products and their derivatives, to treat human diseases including cancer. We analyzed the anticancer effects of T. repens in several cancer cell lines. The phytochemical components of T. repens were first extracted in a methanol solution and then separated into four fractions by ultra-high-performance liquid chromatography. The effects of the total extract and each fraction on cancer cell proliferation were analyzed by MTT assay and Western blotting. T. repens and, more robustly, its isoflavonoid-rich fraction showed high cytotoxic effects in chronic myelogenous leukemia (CML) K562 cells, with IC50 values of 1.67 and 0.092 mg/mL, respectively. The block of cell growth was associated with a total inhibition of BCR-ABL/STAT5 and activation of the p38 signaling pathways. In contrast, these strongly cytotoxic effects did not occur in normal cells. Our findings suggest that the development of novel compounds derived from phytochemical molecules contained in Trifolium might lead to the identification of new therapeutic agents active against CML.
Databáze: OpenAIRE
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