Toxicity of the readily leachable fraction of urban PM2.5 to human lung epithelial cells: Role of soluble metals

Autor: Giovanna Armiento, Maria Rita Montereali, Simonetta Palleschi, Elisa Nardi, Simona Mazziotti Tagliani, Antonio Gianfagna, Barbara Rossi, Leopoldo Silvestroni, Marco Inglessis
Přispěvatelé: Nardi, E., Montereali, M. R., Armiento, G.
Rok vydání: 2018
Předmět:
0301 basic medicine
Health
Toxicology and Mutagenesis
environmental exposure
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Water-soluble compound
oxidative stress
toxicology and mutagenesis
humans
Homocysteine
chemistry.chemical_classification
seasons
Aqueous solution
health
cell line
General Medicine
particle size
urban PM2.5
Pollution
Environmental chemistry
visual_art
Thiol
visual_art.visual_art_medium
Environmental Engineering
oxidation-reduction
metals
Thiol oxidation
chemistry
complex mixtures
transition metals
lung
Metal
03 medical and health sciences
water-soluble compounds
air pollutants
epithelial cells
particulate matter
environmental engineering
environmental chemistry
pollution
health
toxicology and mutagenesis
Extracellular
medicine
Environmental Chemistry
Viability assay
0105 earth and related environmental sciences
Public Health
Environmental and Occupational Health
General Chemistry
Urban PM2.5
Oxidative stress
Water-soluble compounds
Transition metals
030104 developmental biology
Oxidative stre
Leaching (metallurgy)
Cysteine
Zdroj: Chemosphere. 196:35-44
ISSN: 0045-6535
DOI: 10.1016/j.chemosphere.2017.12.147
Popis: Fine airborne particulate matter (PM2.5) has been repeatedly associated with adverse health effects in humans. The PM2.5 soluble fraction, and soluble metals in particular, are thought to cause lung damage. Literature data, however, are not consistent and the role of leachable metals is still under debate. In this study, Winter and Summer urban PM2.5 aqueous extracts, obtained by using a bio-compatible solution and different contact times at 37 °C, were used to investigate cytotoxic effects of PM2.5 in cultured lung epithelial cells (A549) and the role played by the leachable metals Cu, Fe, Zn, Ni, Pb and Cd. Cell viability and migration, as well as intracellular glutathione, extracellular cysteine, cysteinylglycine and homocysteine concentrations, were evaluated in cells challenged with both PM2.5 extracts before and after ultrafiltration and artificial metal ion solutions mimicking the metal composition of the genuine extracts. The thiol oxidative potential was also evaluated by an abiotic test. Results demonstrate that PM2.5 bioactive components were released within minutes of PM2.5 interaction with the leaching solution. Among these are i) low MW (
Databáze: OpenAIRE
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