Genetic dereplication driven discovery of a tricinoloniol acid biosynthetic pathway in Trichoderma hypoxylon
| Autor: | Wen-Bing Yin, Huan Liu, Li-Xia Guo, Jinwei Ren, Yu-Han Pu, Erwei Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
biology
Chemistry Hypoxylon Organic Chemistry Trichothecene Molecular Conformation Computational biology biology.organism_classification Biochemistry Transcriptome Lactones chemistry.chemical_compound Biosynthesis Hypocreales Gene expression Spiro Compounds Physical and Theoretical Chemistry Trichoderma hypoxylon Sesquiterpenes Gene |
| Zdroj: | Organic & Biomolecular Chemistry. 18:5344-5348 |
| ISSN: | 1477-0539 1477-0520 |
| DOI: | 10.1039/d0ob01202e |
| Popis: | A genetic dereplication approach in combination with differential gene expression led to the discovery of three new sesquiterpenes, tricinoloniol acids (TRAs) A-C (1-3) and the known fusidilactone A (4) from T. hypoxylon. Comparative transcriptomic analysis and targeted deletion identified the biosynthetic route for TRAs. Our results demonstrate an alternative application of the genetic dereplication method for exploring the biosynthesis of cryptic secondary metabolites (SMs), which utilizes the coordinated expression of trichothecene (tri) and tra cluster genes. |
| Databáze: | OpenAIRE |
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