Prazosin determination by high-pressure liquid chromatography using flourescence detection
| Autor: | Peter J. Meffin, Yin Gail Yee, Peter Rubin |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Quinidine
chemistry.chemical_classification Chromatography Coefficient of variation Organic Chemistry Prazosin General Medicine Sulfonic acid Biochemistry High-performance liquid chromatography Analytical Chemistry Cold Temperature Pentane chemistry.chemical_compound Spectrometry Fluorescence Drug Stability chemistry Quinazolines medicine Humans Sample preparation Chromatography High Pressure Liquid Whole blood medicine.drug |
| Zdroj: | Journal of Chromatography A. 172:313-318 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(00)90967-1 |
| Popis: | A high-pressure liquid chromatographic procedure using fluorescence detection for the analysis of prazosin in whole blood and plasma is described. It employs a simple and rapid method of sample preparation. Prazosin and an internal standard are chromatographed as ion-pairs with pentane sulfonic acid. The method is sensitive and reproducible with accurate detection as low as 0.2 ng/ml in whole blood and 0.5 ng/ml in plasma with a coefficient of variation of 5.6% and 5.4% respectively. If propranolol or quinidine is present, modifications of chromatographic conditions are used which separate prazosin from these two drugs. Prazosin is stable when frozen for a long period or refrigerated for short periods. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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