AGL9: A Novel Hepatoprotective Peptide from the Larvae of Edible Insects Alleviates Obesity-Induced Hepatic Inflammation by Regulating AMPK/Nrf2 Signaling
| Autor: | Jihye Kim, Byung-Gyu Kim, Eun-Kyung Kim, Bokyung Lee, Sung Mun Bae, Meiqi Fan, Yujiao Tang, Young-Jin Choi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
nonalcoholic fatty liver disease
medicine.medical_specialty Health (social science) Plant Science TP1-1185 medicine.disease_cause hepatic lipid metabolism Health Professions (miscellaneous) Microbiology Article Internal medicine Nonalcoholic fatty liver disease medicine chemistry.chemical_classification biology Adiponectin Chemistry Glutathione peroxidase Chemical technology AMPK medicine.disease AGL9 Fatty acid synthase Endocrinology inflammation biology.protein Steatosis Oxidative stress hepatoprotective peptide Food Science Lipoprotein |
| Zdroj: | Foods, Vol 10, Iss 1973, p 1973 (2021) Foods Volume 10 Issue 9 |
| ISSN: | 2304-8158 |
| Popis: | In this study, we investigated the anti-obesity properties of the novel peptide Ala-Gly-Leu-Gln-Phe-Pro-Val-Gly-Arg (AGL9), isolated from the enzymatic hydrolysate of Allomyrinadichotoma larvae. To investigate the preventive effects of AGL9 against hepatic steatosis and its possible mechanisms of action, we established an nonalcoholic fatty liver disease (NAFLD) model by feeding C57BL/6 mice a high-fat diet. NAFLD mice were administered 100 mg/kg AGL9 and 60 mg/kg orlistat via gavage (10 mL/kg) for 5 weeks, followed by the collection of blood and liver tissues. We found that AGL9 normalized the levels of serum alanine aminotransferase, aspartate aminotransferase, triglyceride, total cholesterol, high-density lipoprotein, very low-density lipoprotein (LDL)/LDL, adiponectin, and leptin in these mice. Additionally, AGL9 activated the protein-level expression of 5′ AMP-activated protein kinase and acetyl-CoA carboxylase phosphorylation and the transcript-level expression of sterol regulatory element-binding protein-1c, fatty acid synthase, superoxide dismutase, glutathione peroxidase, glucocorticoid receptor, nuclear respiratory factor 2, tumor necrosis factor-α, interleukin-1β, interleukin-6, and monocyte chemoattractant protein-1 in hepatocytes. These results showed that AGL9 exhibited hepatoprotective effects by attenuating lipid deposition, oxidative stress, and inflammation via inhibition of AMPK/Nrf2 signaling, thereby reducing the production of hepatic proinflammatory mediators and indicating AGL9 as a potential therapeutic strategy for NAFLD. |
| Databáze: | OpenAIRE |
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