Conserved regulation of the lymphocyte-specific expression oflckin the Fugu and mammals
| Autor: | Byrappa Venkatesh, Sydney Brenner, Sathivel Ponniah, Yue Wang, Wai Ho Yap, Alice Tay, Y. H. Tan, Chih-Fong Chou |
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| Rok vydání: | 2002 |
| Předmět: |
T cell
Green Fluorescent Proteins Molecular Sequence Data Mice Transgenic Biology Takifugu Conserved sequence Mice Genes Reporter Transcription (biology) medicine Animals Humans Amino Acid Sequence Promoter Regions Genetic Gene Conserved Sequence Cell Line Transformed Mammals Regulation of gene expression Multidisciplinary Sequence Homology Amino Acid Fugu Gene Expression Profiling fungi Promoter Biological Sciences Cosmids biology.organism_classification Molecular biology Luminescent Proteins medicine.anatomical_structure Gene Expression Regulation Lymphocyte Specific Protein Tyrosine Kinase p56(lck) |
| Zdroj: | Proceedings of the National Academy of Sciences. 99:2936-2941 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.032680599 |
| Popis: | Thelckgene encodes a lymphocyte-specific protein-tyrosine kinase that is implicated in T cell maturation and signaling. In mammals, the transcription of thelckgene is regulated by two independent promoters, the proximal promoter, which is active in thymocytes, and the distal promoter, which dominates in mature T cells. In the human and mouselckgene loci, the two promoter elements are separated by at least 40 kb and 10 kb, respectively. In this study, we have cloned and sequenced 60 kb from the pufferfish (Fugu rubripes)lcklocus. The promoter region of the Fugulckspans only 4.2 kb and contains a proximal and a distal promoter in the 2.3-kb region adjacent to the coding sequence. By generating transgenic mice, we have demonstrated that the compact promoter of the Fugulckcontains regulatory elements that direct expression to lymphoid organs of mice. We were able to localize the regulatory elements to a short region of 830 bp without losing specificity to cultured human T cell line. These results show that the basic mechanisms that mediate lymphocyte-specific expression are conserved between teleosts and mammals. The short promoter of the Fugulckisolated by us offers a powerful tool for labeling T cells, targeting expression, and manipulating T cell activity in fishes as well as in mammals. |
| Databáze: | OpenAIRE |
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